对于同源的碳氧乙酶的比较表达特征
Fengshui Yang1, Yujie Li1,2, Mengyuan Gao1,2
1Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China.
Frontiers in physiology
|June 9, 2023
概括
这项研究比较了两种相关的物种中的昆虫碳素化酶 (CXEs),揭示了不同的表达模式. 结果提供了CXE功能和环境友好的害虫控制策略的潜力.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 昆虫的碳氧化酶 (CXEs) 对于解毒和感官过程至关重要.
- 有限的数据存在CXEs在密切相关的物种.
- 了解CXE的多样性是开发可持续害虫管理的关键.
研究的目的:
- 为了比较 ortologous CXE 基因 (EoblCXE14 和 EgriCXE14) 在两个兄弟的几何物种 Ectropis obliqua 和 Ectropis grisescens 的表达特征.
- 研究这些CXE的功能多样性.
- 为了确定茶叶几何虫害控制的潜在目标.
主要方法:
- 基因克隆和对EoblCXE14和EgriCXE14的序列分析.
- 遗传学分析以确定进化关系.
- 定量实时聚合酶连锁反应 (qRT-PCR) 用于比较不同发育阶段和组织的基因表达水平.
- 检查潜在的沃尔巴基亚对CXE14的影响.
主要成果:
- EoblCXE14和EgriCXE14共享保存的图案和家族遗传关系.
- EoblCXE14主要表达在E. obliqua幼虫中,而EgriCXE14在E. grisescens中丰富,跨越多个阶段.
- 这两种CXE都在幼虫的中肠高度表达,EoblCXE14在E. obliqua.中表达明显更高.
- 研究了共生细菌沃尔巴基亚对CXE14的影响.
结论:
- 在兄弟的几何蝶中对正确的CXE基因的比较表达分析提供了新的见解.
- 这两种物种之间,EoblCXE14和EgriCXE14的表达模式显著不同.
- 这些发现有助于理解CXE功能,并为茶几何虫害管理提供潜在的目标.
相关概念视频
01:13Carboxylic Acids to Esters: Acid-Catalyzed (Fischer) Esterification Mechanism
8.1K
Carboxylic acids react with alcohols to yield esters via an acid-catalyzed condensation reaction called Fischer esterification. This is a nucleophilic acyl substitution reaction that proceeds via a tetrahedral intermediate, where a water molecule is eliminated as the leaving group.
8.1K
01:14Alkylation of β-Diester Enolates: Malonic Ester Synthesis
3.5K
Malonic ester synthesis is a method to obtain α substituted carboxylic acids from ꞵ-diesters such as diethyl malonate and alkyl halides.
3.5K
01:20Carboxylic Acids to Esters: Acid-Catalyzed (Fischer) Esterification Overview
18.3K
The Fischer esterification reaction was developed by the German chemist Emil Fischer in 1895. It is a condensation reaction between carboxylic acids and alcohols in an acidic medium to give esters and water.
18.3K
01:19Allosteric Proteins-ATCase
5.8K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.8K
01:58Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
01:25Esters to Carboxylic Acids: Saponification
4.6K
Esters can be hydrolyzed to carboxylic acids under acidic or basic conditions. Base-promoted hydrolysis of esters is a nucleophilic acyl substitution reaction in which esters react with an aqueous base, followed by an acid to give carboxylic acids. This reaction is also known as saponification because it forms the basis for making soaps from fats.
The reaction requires a base in stoichiometric amounts, which participates in the reaction and is not regenerated later. So, the base acts as a...
The reaction requires a base in stoichiometric amounts, which participates in the reaction and is not regenerated later. So, the base acts as a...
4.6K