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Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

69
Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
69

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Isolating Free Carbenes, their Mixed Dimers and Organic Radicals
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从贝贝林中分离出来的贝贝林.

R M Gamini Rajapakse1, Benjamin R Horrocks2, A U Malikaramage1

  • 1Department of Chemistry, Faculty of Science, University of Peradeniya Peradeniya 20400 Sri Lanka rmgr1521961@gmail.com.

RSC advances
|June 9, 2023
PubMed
概括
此摘要是机器生成的。

来自树的柏柏林被用光谱学和量子计算研究. 它的电子特性适合创建新的捐赠-接受聚合物.

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科学领域:

  • 摄影化学的使用.
  • 计算化学计算化学
  • 材料科学 材料科学 材料科学

背景情况:

  • 柏柏林是来自Coscinium fenestratum (黄树) 的天然化物,具有有趣的光物理特性.
  • 了解柏柏林的电子结构对于其在先进材料中的应用至关重要.
  • 之前的研究已经探索了柏柏林的生物活动,但其详细的电子和电化学行为需要进一步调查.

研究的目的:

  • 描述不同介质中的柏柏林的UV-Vis吸收和发射光谱.
  • 以计算方式研究柏柏林的电子转换和电荷转移特性.
  • 确定电化学差距并评估其聚合成捐赠-接受材料的潜力.

主要方法:

  • 使用列色谱从Coscinium fenestratum中提取和净化柏柏林.
  • 在乙和水溶液中进行UV-Vis吸收光谱.
  • 时间依赖密度函数理论 (TD-DFT) 用B3LYP函数进行计算.
  • 微电极电压测量用于电化学间隙测定.
  • 使用cc-pVTZ基础设置与B3LYP,CAM-B3LYP和wB97XD函数的量子化学计算.

主要成果:

  • TD-DFT计算准确地重现了柏柏林吸收和排放光谱的主要特征.
  • 电子过渡涉及电子密度从甲基二氧化烯环转移到异诺部分.
  • 电化学间隙从电压计估计为2.64V,显示与量子化学计算的良好一致.
  • 计算表明,贝贝林的基质分离中脱局域自旋密度.

结论:

  • 柏柏林表现出与捐赠者-接受者系统一致的电子特性.
  • 计算的电子转换和电化学差距为材料设计提供了有价值的数据.
  • 柏柏林是通过氧化或共聚合合成新型捐助-接受聚合物材料的有希望的候选物.