循环和细胞外囊泡衍生的微RNA作为骨相关疾病中的生物标志物
Julika Huber1,2,3,4, Michael T Longaker1,2,3,5, Natalina Quarto1,2,3
1Hagey Laboratory for Pediatric Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, United States.
Frontiers in endocrinology
|June 9, 2023
概括
循环中的微RNA (miRNA) 是骨质疏松症和骨肉瘤等骨疾病的有希望的生物标志物. 这些易于获得的分子提供了非侵入性诊断和预后潜力,反映了疾病状态和治疗反应.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 生物标志物发现发现
背景情况:
- 微RNA (miRNA) 是小型非编码RNA,在转录后调节基因表达.
- 在细胞外囊中受保护的循环miRNAs是可访问的,疾病特异性生物标志物.
- miRNAs 影响骨代谢,影响骨质生成.
研究的目的:
- 审查循环和细胞外囊泡衍生的miRNAs作为骨相关疾病中的生物标志物的作用.
- 专注于骨质疏松症和骨质肉瘤.
- 讨论miRNA生物学,生物标记物类型和未来的前景.
主要方法:
- 在骨疾病中对miRNA进行全面的文献搜索.
- 对miRNA历史,生物学和生物标志物应用的审查.
- 在骨质疏松症和骨髓瘤中分析miRNA特征.
主要成果:
- 循环中的miRNAs对于骨疾病的非侵入性诊断和预后非常有价值.
- miRNA签名可以表明疾病状态,进展和治疗反应.
- 细胞外囊泡衍生的miRNAs提供了增强的稳定性和特异性.
结论:
- 循环的miRNAs在诊断和监测骨疾病方面取得了重大进展.
- 需要进一步的研究来克服局限性,并充分实现其临床潜力.
- miRNAs是骨生物学中的关键调节者,具有诊断和治疗意义.
相关概念视频
01:36Overview of Exosomes
2.8K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.8K
01:22MicroRNAs
21.4K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.4K