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多抗药性ST648的基因组特征

Xianhong He¹, Liwei Xu¹, Hangdong Dai¹

¹Department of Clinical Laboratory, The Third People's Hospital of Xiaoshan, Hangzhou, People's Republic of China.

Infection and drug resistance

|June 9, 2023

概括

这项研究详细介绍了一种来自中国的抗药性大肠杆菌菌株,该菌株携带了blaKPC-2和blaCTX-M-15基因. 了解其基因组特征,可以了解耐卡巴胺的肠杆菌传播和抵抗机制.

科学领域:

微生物学微生物学
基因组学就是基因组学.
传染性疾病传染性疾病

背景情况:

耐卡巴胺肠道细菌 (CRE) 构成了全球严重的健康威胁.
许多抗菌剂的有效性受到CRE传播的影响.
这项研究的重点是来自中国的多抗药性大肠杆菌分离物.

研究的目的:

描述一种耐多药性大肠杆菌的基因组特征.
为了确定特定抗微生物耐药性基因 (blaKPC-2和blaCTX-M-15) 的存在和位置.
了解这种临床隔离物中抗菌素耐药性的遗传基础.

主要方法:

使用牛津纳米孔MiniON和Illumina NovaSeq 6000进行全基因组测序.
序列的 De novo 组装可以读取.
在多位元序列类型 (MLST),抗菌素耐药性基因和等离子体复制体类型的分析中.
核心基因组单核酸多态 (cgSNP) 与相关菌株的比较.

主要成果:

这种分离物 (大肠杆菌488,ST648) 对多种抗生素,包括卡巴类药物表现出耐药性.
完整的基因组序列显示了一个染色体和十个质粒.
关键的耐药基因,blaKPC-2和blaCTX-M-15,分别在Inci1等离子体和染色体上被确定.
隔离物与其他中国大肠杆菌菌株有密切的遗传关系,SNP差异很小.

结论:

这是中国首次报告一种ST648大肠杆菌分离物,其中含有blaKPC-2和blaCTX-M-15基因.
这些发现为CRE的遗传构成和抵抗机制提供了关键的见解.
了解这些特征对于在临床环境中追踪和控制抗卡巴胺抗性肠道细菌的传播至关重要.

关键词:

埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.在blaCTX-M-15中使用.在blaKPC-2中使用.具有多种耐药性的药物.整个基因组的测序.

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