在口腔状细胞癌中切换卡德林:一项临床病理学研究
Ahmed Noaman Ali1, Shoukria Mohammed Ghoneim1, Esam Rashwan Ahmed2
1Oral Pathology, Oral Pathology Department, Faculty of Dentistry, Tanta University, Egypt.
Journal of oral biology and craniofacial research
|June 9, 2023
概括
卡德林切换,涉及降低E-卡德林和增加N-卡德林,对于口腔状细胞癌 (OSCC) 的进展和转移至关重要. 这种分子转移是OSCC发展的重要指标.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 口腔状细胞癌 (OSCC) 是一种普遍存在的全球性恶性瘤.
- 表皮-介质细胞过渡 (EMT) 是推动OSCC进展的关键机制.
- 卡德林切换,以降低E-卡德林和升高N-卡德林为特征,是EMT的核心.
研究的目的:
- 阐明卡德林切换在口腔状细胞癌 (OSCC) 中的作用.
- 调查卡德林切换与OSCC进展 (包括转移) 之间的相关性.
主要方法:
- 在30个OSCC组织样本中对E-cadherin和N-cadherin进行免疫组织化学染色.
- 使用RT-PCR对OSCC细胞系 (SCC-15/SCC-25) 中E-cadherin和N-cadherinmRNA表达的分析.
- 在细胞培养中使用F-12K介质诱导EMT.
主要成果:
- 卡德林切换在OSCC中显示出与他的病理学等级的显著相关性.
- 在转移性OSCC中观察到N-cadherin升高和E-cadherin降低.
- 在EMT期间在OSCC细胞系中的E-cadherin和N-cadherin的mRNA基因表达中发现了显著的相关性.
结论:
- 卡德林切换是OSCC内EMT过程中的一个关键事件.
- 卡德林切换是研究OSCC进展的一个有价值的标志物.
- 卡德林切换显著促进OSCC的入侵和转移.
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