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在囊性纤维化肺微环境中的Exophiala dermatitidis宿主进化中

Tania Kurbessoian¹, Daniel Murante², Alex Crocker²

¹Department of Microbiology and Plant Pathology and Institute of Integrative Genome Biology, University of California Riverside, Riverside, CA 92521, USA.

G3 (Bethesda, Md.)

|June 9, 2023

概括

囊性纤维化 (CF) 患者患有Exophiala dermatitidis引起的持续性真菌感染. 基因组分析揭示了单个患者内的多个,基因相似的血统,突出了慢性真菌感染中的人口异质性.

科学领域:

医学真菌学医学真菌学
基因组学就是基因组学.
传染性疾病传染性疾病

背景情况:

患有囊性纤维化 (CF) 的人经历慢性肺部感染,通常涉及像Exophiala dermatitidis这样的真菌.
了解这些真菌病原体的遗传多样性和持久性对于管理CF肺部疾病至关重要.

研究的目的:

为了分析Exophiala dermatitidis从囊性纤维化患者中分离的种群基因组学.
调查CF肺环境中的真菌血统的遗传关系,分歧和潜在的持久性.

主要方法:

使用纳米孔技术对一个Exophiala dermatitidis分离物的全基因组测序.
使用单核酸多态和插入删除变体检测对23个分离物的比较基因组分析.
种群基因组学和遗传学分析以推断进化关系并识别不同的类.

主要成果:

在CF肺部种群中,发现了三种不同的Exophiala dermatitidis菌株,它们的突变率各不相同.
隔离物高度相关,表明最近的分歧,所有都属于MAT 1-1交配类型,没有再组合的证据.
遗传学分析揭示了从早期和晚期的时间点分离的持久血统,以及与运输,新陈代谢和DNA修复相关的基因中的类特异性功能变异.

结论:

在CF肺部的Exophiala dermatitidis分离物中存在持续的种群异质性.
基因组和表型变异有助于这种真菌在生物体中的适应性和持久性.
研究随着时间的推移而发生的真菌病原体进化为慢性感染中缓慢生长的真菌的生理学提供了洞察力.

关键词:

在外皮炎皮肤炎.黑酵母是黑色酵母的一种.囊性纤维化病的发生.人口基因组学人口基因组学

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