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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.4K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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相关实验视频

Updated: Jul 27, 2025

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
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通过强大的扭曲校正进行原子分辨率低温4D-STEM成像.

Jacob Smith1,2, Zhennan Huang2, Wenpei Gao1

  • 1Department of Materials Science and Engineering, North Carolina State University, Raleigh, North Carolina 27695, United States of America.

ACS nano
|June 9, 2023
PubMed
概括

我们开发了一个新的算法来纠正冷四维扫描传输电子显微镜 (4D-STEM) 数据中的扭曲. 这种方法增强了量子材料的原子尺度成像,使其性能能够更准确地分析.

关键词:
在4D-STEM中,有关转换的相似转换.低温产生的冷物质.扭曲纠正 纠正 扭曲纠正传输电子显微镜 传输电子显微镜三角化的三角化

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科学领域:

  • 材料科学 材料科学 材料科学
  • 物理 物理学 物理
  • 电子显微镜电子显微镜

背景情况:

  • 低温四维扫描传输电子显微镜 (4D-STEM) 能够在原子尺度上对量子材料进行表征.
  • 目前的局限性包括冷阶段和影响数据质量的电子不稳定性.

研究的目的:

  • 开发一种算法来纠正冷4D-STEM数据中的复杂扭曲.
  • 提高4D-STEM在量子材料研究中的可靠性和适用性.

主要方法:

  • 使用非刚性注册来识别4D-STEM数据集中的局部扭曲.
  • 应用了亲缘变换来准确纠正扭曲.
  • 在真实空间和相互空间中确保最小的信息损失.

主要成果:

  • 成功地纠正了原子分辨率冷4D-STEM数据中的复杂扭曲.
  • 从扭曲的数据集中准确重建样本信息.
  • 证明了在飞行分析中的计算效率和速度.

结论:

  • 开发的算法有效地克服了冷4D-STEM数据采集的局限性.
  • 这种方法有助于在原子尺度上对量子材料进行先进的现场研究.
  • 该技术在计算上是廉价的,适合实时数据处理.