通过向各种细菌扩展模块化工具包,系统地进行部分转移
Kevin W Keating1, Eric M Young1
1Life Sciences and Bioengineering Center, Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, Massachusetts 01605, United States.
ACS synthetic biology
|June 9, 2023
概括
合成生物学部分可以在各个生物体中转移,从而使基因表达可预测. 这项研究量化了多个宿主中的部分行为,为跨生物体工程创建了一个查找表.
科学领域:
- 合成生物学 合成生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 为每个宿主生物体开发新的生物部分是不切实际的.
- 已知基因表达部分是可转移的,但关于这种可转移性的定量数据是有限的.
研究的目的:
- 在多个宿主生物体中系统量化标准化一组合成生物学部件的行为.
- 建立一种方法来概括模块化部分的集合超出单个宿主物种.
主要方法:
- 开发了一个与CIDAR零件集合兼容的广域宿主 (BHR) 质粒系统 (openCIDAR).
- 测试的DNA构造跨越了四个Pseudomonadota物种:大肠杆菌,小肠杆菌,Cupriavidus necator和Komagataeibacter nataicola.
- 使用等效素分子 (MEFL) 量化部分性能,并应用线性回归来识别分离的部分.
主要成果:
- 通过CIDAR的部分,在所有测试的生物体中实现了分级基因表达,表达趋势类似,但平均水平不同.
- 需要一个查找表来翻译主机之间的设计,以实现一致的MEFL.
- 与其他宿主相比,促进体J23100在Komagataeibacter nataicola中表现出明显不同的行为.
结论:
- 开放CIDAR系统允许对多种蛋白质菌中的CIDAR兼容部件进行评估,将合成生物学部件集概括为.
- 这种方法加速了各种应用的不同物种的工程工作.
- 一些标准化的零件集可能会跨越生命之树.
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