探索不同涂层纳米颗粒的抗菌活性和细菌介导的全热转变
Miguel A Ruiz-Fresneda1, Sebastian Schaefer1,2, René Hübner3
1Department of Microbiology, University of Granada, Campus Fuentenueva, 18071 Granada, Spain.
ACS applied materials & interfaces
|June 9, 2023
概括
纳米颗粒 (SeNPs) 显示出强大的抗菌活性对抗阴性和阳性细菌,为银纳米颗粒 (AgNPs) 提供了一个有希望的替代品. 这些SeNP还表明了结晶纳米粒子生物修复和绿色合成的潜力.
科学领域:
- 纳米技术 纳米技术
- 抗微生物药物是一种抗菌剂.
- 环境科学环境科学
背景情况:
- 金属纳米颗粒 (NPs),特别是银纳米颗粒 (AgNPs),是确立的抗菌剂.
- 人们越来越担心抗生素耐药性,因此需要替代生物杀菌化合物.
- 纳米颗粒 (SeNPs) 正在成为有效的抗菌剂,具有超出AgNPs的潜在应用.
研究的目的:
- 研究和比较不同表面涂层 (BSA,奇托桑,未定义) 的纳米颗粒 (SeNPs) 与Stenotrophomonas bentonitica (克拉姆阴性) 和Lysinibacillus sphaericus (克拉姆阳性) 的抗菌活性.
- 评估SeNPs与银纳米颗粒 (AgNPs) 的比较疗效.
- 探索SeNPs在生物修复和结晶纳米粒子绿色合成中的潜力.
主要方法:
- 合成和表征具有相似物理性质 (大小,形状,结构) 但不同的表面电荷的SeNP和AgNP.
- 使用微热量计和流细胞计来评估抗菌活性,以测量细胞活力和生长抑制.
- 量化反应性氧物种 (ROS) 生产和DNA降解.
- 使用电子显微镜分析细菌细胞的NP转化.
主要成果:
- 所有测试的NP都对细菌细胞生长和活力产生了负面影响.
- 未定义涂层的SeNP表现出最高的细菌细胞死亡率 (85-91%).
- 桑涂层和未定义的SeNPs诱导了显著的活性氧物种 (ROS) 生产.
- 未定义-SeNP在降解细菌DNA方面最有效 (近80%).
- 细菌细胞将无形的SeNPs转化为晶体形式,表明生物修复和绿色合成的潜力.
结论:
- 纳米颗粒 (SeNPs) 显示出作为抗菌剂的巨大潜力,与AgNPs相当或优于AgNPs.
- SeNPs的表面涂层影响其抗菌功效和ROS生成.
- 细菌观察到的无形SeNPs转化为晶体SeNPs为生物修复和绿色合成应用提供了新的途径.
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