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Jane Marian Luis1, Hillary L Mehl1,2, Dianne Plewa3
1Tidewater Agricultural Research and Extension Center, Virginia Tech, Suffolk, VA 23437.
Phytopathology
|June 9, 2023
概括
这项研究研究了与玉米焦油斑鱼眼病变相关的真菌. 大多数分离物被确定为Fusarium物种,这表明以前的Microdochium maydis.dis的错误识别.
科学领域:
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
- 农业科学 农业科学
背景情况:
- 由Phyllachora maydis引起的焦油斑是一种新兴的玉米疾病.
- 被称为"鱼眼"的死角病变围绕着P. maydis stromata.
- 这些病变以前被归因于Microdochium maydis,最近的文档有限.
研究的目的:
- 为了识别与围绕P. maydis.周围的鱼眼病变相关的Microdochium类真菌.
- 为了澄清玉米焦油斑疾病中鱼眼症状的致病因子.
主要方法:
- 从墨西哥,伊利诺伊州和威斯康星州收集了带有鱼眼病变的玉米叶样本.
- 从病变中分离和培养的真菌,包括被认为是墨西哥M. maydis的分离物.
- 使用多基因遗传学分析 (ITS,TEF1-α,RPB1,RPB2) 进行隔离物鉴定.
主要成果:
- 获得了101种微多奇/类分离物;91%的分离物被确定为类. 通过ITS测序.通过ITS测序.
- 遗传学分析证实了所有分离物聚集在Fusarium系内,与Microdochium不同.
- 来自墨西哥的Fusarium分离物属于F. incarnatum-equiseti复合物;美国的分离物主要属于F. sambucinum复合物.
结论:
- 造成鱼眼病变的真菌主要是Fusarium物种,而不是Microdochium maydis.
- 之前关于M.maydis与这些病变相关的报道可能是由于错误识别造成的.
- 这项研究澄清了玉米焦油斑中鱼眼症状的真菌病因.
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