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在临床和猪产业的临床和猪产业分离物中的1类整体
Nancy Yaneth Flórez-Delgado1,2, Elizabeth Noelia Ubillus1,3, Blanca Perez-Sepulveda4
1Grupo de Microbiología, Instituto Nacional de Salud, Avenida calle 26 No. 51-20 - Zona 6 CAN, Bogotá, D.C., Colombia.
Journal of medical microbiology
|June 9, 2023
概括
1类整合子在哥伦比亚沙门氏菌Typhimurium中广泛存在,与人类和猪分离物中的抗菌素耐药性基因有关. 这项研究强调了它们在传播抗性决定因素中的作用.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 公共卫生 公共卫生
背景情况:
- 沙门氏菌 (Salmonella enterica) 的一个亚种. 输入式血清 Typhimurium (S. Typhimurium) 是导致食物传播疾病和抗菌素耐药性的重要原因.
- 在哥伦比亚,S. Typhimurium是最常见的沙门氏菌血清,在1997-2018年间观察到抗生素耐药性增加.
研究的目的:
- 在哥伦比亚S. Typhimurium分离物中识别1类整体.
- 调查1类整体与其他移动遗传元素的关联.
- 为了确定1类整体和抗菌素耐药性模式之间的关系.
主要方法:
- 来自人类临床和猪样本的442个S. Typhimurium分离物的分析.
- 使用PCR和全基因组测序 (WGS) 检测1类整体子和等离子体不兼容组.
- 使用多位元序列类型 (MLST) 和单核酸多态 (SNP) 距离的系谱分析.
主要成果:
- 在39%的人类临床和22%猪分离物中发现了完整的1类整合子.
- 与抗性基因如dfr7-aac-bla OXA-2相关的Int1-Col1整体是最常见的类型.
- 具有1类整体的分离物对多个抗微生物家族表现出耐药性,其中Inca/C等离子体组是最普遍的.
结论:
- 自1997年以来,IntI1-Col1综合体在哥伦比亚普遍存在,这表明了严重的公共卫生问题.
- 整子,隔离源和移动元素之间存在联系,促进了S. Typhimurium中抗菌素耐药性的传播.
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