氧化还原潜力对Bacillus megaterium聚3-基酸盐的积累的影响
Guilherme de Souza Hassemer1, Lucas Henrique do Nascimento1, Yen-Han Lin2
1Department of Food Engineering, URI Erechim, Av. Sete de Setembro 1621, Erechim, RS, 99709-910, Brazil.
Bioprocess and biosystems engineering
|June 9, 2023
概括
控制氧化降解潜力 (ORP) 优化了Bacillus megaterium中聚3-基酸 (P(3HB)) 的产生. 在0mV保持ORP显著增加了生物质和P(3HB) 产量,突出了ORP.
科学领域:
- 生物技术是生物技术.
- 微生物的新陈代谢
- 生物聚合物生产 生物聚合物生产
背景情况:
- 微生物的新陈代谢受到氧化减少潜力 (ORP) 的影响.
- 控制ORP可以操纵代谢流和酶表达.
- 聚3-基酸盐 (P3HB) 是一种具有多种应用的生物聚合物.
研究的目的:
- 评估氧化降解潜力 (ORP) 对Bacillus megaterium产生的聚3-基酸 (P(3HB)) 产生的影响.
- 确定最佳的ORP水平,以最大限度地提高生物质和P(3HB) 产量.
- 评估ORP在发酵过程中对微生物代谢的影响.
主要方法:
- 在矿物质介质中发酵Bacillus megaterium,并添加农业工业副产品.
- 在1L容器中培养,温度控制 (30°C),动 (500rpm) 和空气流.
- 使用ORP探针和电磁监测和控制氧化降解电位 (ORP).
主要成果:
- 0 mV ORP 的培养物产生了最高的总生物质 (5.00 g/L),相比之下, -20 mV (2.90 g/L) 和 -40 mV (0.53 g/L).
- 在培养48小时后,最大的P(3HB) 与生物质比率达到69.87%,聚合物度降低到0mV ORP以下.
- 培养的pH也影响了生物质和聚合物度,虽然比ORP更少.
结论:
- 氧化降低潜力 (ORP) 显著影响了Bacillus megaterium的新陈代谢和P3HB的产生.
- 保持0mV的ORP对于最大限度地提高生物质和P3HB) 产量至关重要.
- 在各种发酵条件下,ORP控制是优化生物聚合物生产的宝贵工具.
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