分子伴侣mtHSC70-1与DjA30相互作用,以调节Arabidopsis中的雌性性异构体发育和生育能力
Xiaoting Zhai1,2,3,4, Jiaoteng Bai1,2,3, Wenyan Xu1,2,3
1Ministry of Education Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China.
The Plant journal : for cell and molecular biology
|June 9, 2023
概括
线粒体向的热冲击蛋白70 (mtHSC70-1) 和DJA30对于植物生育和雌性性质细胞的发育至关重要,因为它们通过维持胚胎囊中的活性氧物种 (ROS) 恒温.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 阿拉比多的线粒体向热冲击蛋白70 (mtHSC70-1) 在植物生长过程中对呼吸和氧化还原平衡至关重要.
- 植物的生育和体育细胞的发育是由细胞平衡影响的复杂过程.
研究的目的:
- 研究mtHSC70-1在植物生育能力和雌性性质细胞 (FG) 发育中的作用.
- 探索mtHSC70-1与线粒体J蛋白DJA30在游戏生成中的相互作用.
- 阐明反应性氧物种 (ROS) 稳态在FG发育和生育中的作用.
主要方法:
- 在Arabidopsis中基因淘汰和补充.
- 分析雌性性体细胞的发育,包括线粒分裂和核定位.
- 在体内对mtHSC70-1和DJA30之间的相互作用研究.
- 测量呼吸链复合体IV活性和ROS水平.
- 基因操纵以改变ROS水平.
主要成果:
- mtHSC70-1淘汰突变体表现出降低生育能力和FG发育缺陷.
- DjA30突变体表现出类似的FG发育和生育缺陷.
- mtHSC70-1和DJA30在体内相互作用,并在FG中共同表达.
- 突变胚胎囊显示了下调的呼吸链复合体IV活性,导致ROS积累.
- 降低ROS水平在mtHSC70-1突变种中挽救了FG发育和生育能力.
结论:
- mtHSC70-1和DJA30对于在雌性性生殖过程中维持ROS恒温是必不可少的.
- ROS稳态对于胚胎囊成熟和核模式的形成至关重要.
- 适当的ROS平衡会影响游戏细胞和辅助细胞的命运,影响植物的生育能力.
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