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抑制DNA和RNA甲基化扰乱了moso竹的根部发育

Yuxiang Liufu¹, Feihu Xi², Lin Wu¹

¹College of Forestry, Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, No. 15 Shangxiadian Road, Cangshan District, Fuzhou City, Fujian Province 350002, China.

Tree physiology

|June 9, 2023

概括

表观遗传调节者DNA甲基化 (5mC) 和RNA甲基化 (m6A) 影响植物生长. 这项研究揭示了它们在莫索竹根发育中的相互关联作用,影响基因表达和细胞壁合成.

科学领域:

植物生物学植物生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

DNA甲基化 (5mC) 和N6-甲基氨酸 (m6A) 是影响植物发育的关键表观遗传调节剂.
菲洛斯塔奇斯·埃杜利斯 (moso竹) 呈现出迅速增长的特征,这是由于其广泛的根系.
5mC和m6A之间的相互作用,特别是关于m6A在转录后调节中的作用,在P. edulis.中了解得很少.

研究的目的:

调查Phyllostachys edulis根发育中DNA甲基化 (5mC) 和RNA甲基化 (m6A) 之间的关联.
描述抑制m6A和5mC对根形态和基因表达的影响.
探索莫索竹中5mC和m6A之间的潜在相关性.

主要方法:

形态和电子显微镜观察根系表型.
纳米孔直接RNA测序来分析RNA表转录组.
用RNA甲基化抑制剂 (DZnepA) 和DNA甲基化抑制剂 (5-azaC) 的治疗.

主要成果:

抑制剂DZnepA和5-azaC治疗增加了横向根的形成.
在DZnepA治疗后,3'-UTR中的m6A水平降低与改变的基因表达,多化和转录稳定性相关.
5-azaC治疗减少了CG和CHGDNA甲基化,影响了细胞壁合成.
在DZnepA和5-azaC治疗之间差异表达基因的显著重叠表明5mC和m6A之间的联系.

结论:

这项研究提供了关于m6A和5mC在moso竹根发育中的相互关联作用的初步见解.
甲基化抑制会影响根生长,基因表达和细胞壁完整性.
需要进行进一步的研究,以阐明P. edulis. 中m6A和5mC之间的确切机制.

关键词:

菲洛斯塔基斯 (Phyllostachys edulis) 是一个的动物.通过DNA甲基化.这是一种N6-甲基氨酸.替代性的多基化.长非编码RNA是什么意思多种 (A) 尾巴长度尾巴长度

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