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Updated: Aug 18, 2026

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans
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Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Published on: March 17, 2019

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使用C. elegans进行体内亲和力净化蛋白质学和生物化学的优化协议

Muriel Desbois1, Joseph S Pak1, Karla J Opperman1

  • 1Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, WA 98101, USA.

STAR protocols
|June 9, 2023
PubMed
概括

我们优化了Caenorhabditis elegans的体内亲和力净化蛋白质组. 这种方法成功地识别了蛋白质与蛋白质的相互作用和信号网络,有助于生物化学评估体内蛋白质相互作用.

关键词:
质谱测量质量谱测量模型生物模型生物神经科学是一个神经科学.蛋白质生物化学 蛋白质生物化学蛋白质的表达和净化过程蛋白质组学是指蛋白质组学.

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相关概念视频

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...