线粒体表面的高灵敏度生物传感器显示,IP3R通道参与网状Ca2+泄漏到线粒体
Yves Gouriou1,2, Fabrice Gonnot1,2, Mariam Wehbi1,2
1Univ-Lyon CarMeN Laboratory, Inserm U1060, Université Claude Bernard Lyon 1, INRAE, Bron, France.
PloS one
|June 9, 2023
概括
研究人员开发了一种新型,高度敏感的生物传感器,用于线粒体外膜. 这种工具揭示了IP3受体的内源活性,这对于细胞应激期间的信号传递至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 生物物理学的生物物理.
背景情况:
- 基因编码的光蛋白生物传感器跟踪细胞中的离子 (Ca2+) 动态.
- 线粒体相关膜 (MAMs) 是平衡和线粒体功能的关键.
- 以前的Ca2+生物传感器缺乏在外线粒体膜 (OMM) 中测量本源通道活性的灵敏度.
研究的目的:
- 为OMM开发一个高度敏感的Ca2+生物传感器,以量化MAM附近的Ca2+水平.
- 在生理和压力条件下调查OMM的内源道活动.
主要方法:
- 组装一个基于光蛋白的新型比度Ca2+生物传感器.
- 生物传感器在线粒体外膜 (OMM) 表面的表达.
- 测量线粒体相关膜 (MAMs) 中或附近的Ca2+度变化.
主要成果:
- 新的生物传感器在MAM附近检测到较小的Ca2+差异,比前几代更小.
- 在OMM中证明了IP3受体的内生活性.
- 观察到的IP3受体活性,在缺氧和SERCA抑制期间有助于Ca2+泄漏.
结论:
- 开发的生物传感器可以在OMM进行敏感的Ca2+测量.
- IP3受体表现出内源性活动,影响OMM的Ca2+流量.
- 这一发现为信号传递和在压力下线粒体功能提供了新的见解.
相关概念视频
01:27Feedback Regulation of Calcium Concentration
3.4K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.4K
01:16Calmodulin-dependent Signaling
5.2K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.2K