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使用周期性酸-Schiff (PAS) 组织学染色剂检测牙样本中的细菌

Luciano Giardino¹, Luigi Generali², Massimo Del Fabbro³

¹Independent Researcher, 88900 Crotone, Italy.

Micron (Oxford, England : 1993)

|June 9, 2023

概括

周期性酸-Schiff (PAS) 染料有效检测人类牙组织中的细菌,有助于识别牙管道和根通道内的微生物. 这种体内化学方法可以提高在感染牙中的非或弱染色细菌的可视化.

科学领域:

组织化学组织化学
牙科组织学牙科组织学
微生物学微生物学

背景情况:

由糖蛋白和多糖组成的细菌细胞壁在牙组织中可以使用特定的染色技术检测到.
基因化学方法为复杂的生物样本中的微生物提供了可视化的潜力.

研究的目的:

为了研究周期性酸-Schiff (PAS) 染色剂在人类牙组织学样本中检测细菌存在的疗效.
评估PAS染色与扫描电子显微镜 (SEM) 结合使用的实用性,以进行详细的微生物分析.

主要方法:

人类牙样本被处理为脱,脱水,嵌和连续切割.
用PAS体化学方法染色部分,然后在选定的幻灯片上进行SEM分析.
参考细菌菌株 (ATCC) 使用相同的PAS协议进行染色以进行比较.

主要成果:

PAS染色揭示了人类牙管道和牙根管道内的杆状和状结构,表明存在细菌.
SEM分析证实了这些结构的细菌性质,并提供了关于它们活力的见解.
在不同的ATCC细菌菌株中观察到可变的PAS稳定性.

结论:

血清化学染色PAS是一种有价值的工具,用于检测和可视化细菌,特别是非或弱染色物种,在感染的牙组织中.
将PAS染色与SEM染色相结合,为组织学样本中的微生物识别和表征提供了一个全面的方法.
这种方法可以作为诊断牙科感染的其他调查技术的有用补充.

关键词:

细菌的多糖化合物细菌的多糖牙上的牙.葡萄糖蛋白质是一种葡萄糖蛋白质.在 PAS 染色器上,不存在染色.根管道治疗的方法

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