基于在线闭环模式的强极分离介质从药用植物中分离和净化糖化物
Lu Liu1, Haiyan Liu1, Hongyuan Yan1
1College of Pharmaceutical Sciences, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Public Health Safety of Hebei Province, Hebei University, Baoding 071002, China.
Journal of pharmaceutical and biomedical analysis
|June 9, 2023
概括
研究人员开发了新的单体分离介质,以有效地从药用植物中分离活性甘氨酸. 这些在线闭环方法显著提高了治疗剂研究的产量和纯度.
科学领域:
- 分析化学 分析化学
- 自然产品化学 自然产品化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 来自药用植物的天然糖化物对于治疗剂的发现至关重要.
- 复杂的植物基质对糖化物分离和净化构成重大挑战.
- 需要有效的方法来隔离糖化物用于药理学研究.
研究的目的:
- 开发和应用新的功能单立体分离介质,用于在线提取,分离和净化活性糖化物.
- 建立一个简单的程序闭环系统,用于糖化物隔离.
- 为了提高药用植物特定甘油酸的产量和纯度.
主要方法:
- 制造两个功能单体分离介质 (A和S).
- 介质A作为固体相提取吸附剂的应用,用于从Rhei Radix和Rhizoma中分离醇糖化物和Physcion糖化物.
- 介质S作为高性能液体染色学 (HPLC) 静止相的应用,用于从Rheum hotaoense中分离Rhapontin C. Y. Cheng et Kao. Kao.
- 使用在线闭环HPLC系统,集成样品注射,隔离和净化.
主要成果:
- 成功地分离和净化了克里索芬醇葡萄糖化物,菲西昂葡萄糖化物和Rhapontin.
- 取得的高产量分别为5.68毫克g-1,1.20毫克g-1和4.76毫克g-1.
- 获得高纯度的所有分离的糖化物产品.
- 与离线方法相比,证明减少了样本损失和改善了恢复.
结论:
- 开发的功能单体分离介质和在线闭环HPLC系统为糖化物隔离提供了有效的方法.
- 这些方法提供了一种简化程序,具有高回收率和纯度,促进了天然糖化物的药理研究.
- 制造的介质显示出从复杂的天然产品样本中分析和净化活性成分的巨大潜力.
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