维生素,一个循环表达的离子通道,保护石碑以赋予球根抵抗力
Wei Wang1, Li Qin2, Wenjing Zhang3
1State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China; Hainan Yazhou Bay Seed Laboratory, Sanya 572025, China.
Cell
|June 9, 2023
概括
研究人员发现了WeiTsing (WTS),一种新型基因在植物中赋予广泛的根抗性. 该基因编码了一种可透的离子通道,对激活植物对Plasmodiophora brassicae (Pb) 感染的免疫反应至关重要.
科学领域:
- 植物病理学
- 分子生物学
- 遗传学
背景情况:
- 植物根容易受到致病微生物的影响,导致作物损失很大.
- 由Plasmodiophora brassicae (Pb) 引起的球根病在全球范围内摧毁了十字植物作物.
研究的目的:
- 识别和描述一种新型基因,使其对根病具有抵抗力.
- 阐明植物对Plasmodiophora brassicae免疫的分子机制.
主要方法:
- 从Arabidopsis中分离和表征WTS基因.
- 针对Pb感染的WTS表达分析.
- 具有WTS基因的转基因Brassica napus的产生
- 用于结构分析的冷电子显微镜 (cryo-EM).
- 电生理学来确定通道活动.
- 结构引导的突变发生,以评估通道活动的作用.
主要成果:
- 在Pb感染时,WTS基因在周环中被转录激活,防止病原体进入星座.
- 具有WTS的转基因Brassica napus对Pb表现出强烈的耐药性.
- WTS编码在内细胞网 (ER) 中局部化的小蛋白质,并诱导免疫反应.
- 克里奥-EM检测发现一个独特的体结构, 中央孔隙作为透性离子通道.
- WTS的通道活动对于触发植物防御是必不可少的.
结论:
- WTS是一种广泛的树根抗性基因.
- WTS作为一个离子通道,类似于抵抗体,对于启动周环中的免疫信号至关重要.
- 这些发现为开发抗病作物提供了新的目标.
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