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心脏肌纤维形成是由分子伴侣UNC45B的空间时间调节的

Serena Huei-An Lu¹, Yi-Hsuan Wu¹, Liang-Yu Su¹

¹Department of Life Science, National Taiwan University, Taipei 10617, Taiwan.

Stem cell reports

|June 9, 2023

概括

分子陪伴者UNC45B对于心肌收缩至关重要,通过控制原生体形成和蛋白质相互作用来调节体组合. 它的缺失严重损害了心肌细胞收缩性和肌纤维形成.

科学领域:

心血管生物学心血管生物学
肌肉生理学肌肉生理学
干细胞生物学干细胞生物学

背景情况:

癌症瘤功能障碍是心肌病的基础,这是一个主要的全球健康问题.
准确的分子机制,控制在心肌细胞中的沙科默尔组装尚未完全理解.

研究的目的:

阐明参与心脏肌纤维形成的蛋白质的时空调节.
调查分子伴侣UNC45B在肉瘤形成和心肌细胞功能中的作用.

主要方法:

使用人类胚胎干细胞 (hESC) 衍生的心肌细胞 (CMs).
进行了UNC45B的淘汰赛实验和表型分析.
检查了蛋白质的共同表达,分布和相互作用 (例如,UNC45B,KINDLIN2,MYH6,ACTN2,F-ACTIN).

主要成果:

UNC45B与KINDLIN2 (protocostamere标记物) 共同表达,后来与MYH6.6重叠.
在UNC45B中,KNOCK-OUTCMs完全丧失了收缩能力.
损坏的原生体形成会破坏ACTN2结合,抑制F-ACTIN聚合,并导致MYH6降解.

结论:

UNC45B对于心脏肌纤维形成至关重要,通过KIND2调节调解原生细菌形成.
UNC45B的时空相互作用对于适当的瘤细胞组合和心肌细胞功能至关重要.
了解UNC45B的作用为心肌病和潜在的治疗点提供了洞察力.

关键词:

这就是CRISPR/Cas9技术.这种类型的瘤是sarcomere.心脏肌纤维细胞发生.这是心肌细胞 (cardiomyocyte).分子陪伴者分子陪伴者一个原始的costamerecostamere.

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