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在可持续的生物反应介质中,改进了用素增值的素转化为利胺的生物转化

Qi Li¹, Ruiying Gao², Yucheng Li³

¹School of Pharmacy, National-Local Joint Engineering Research Center of Biomass Refining and High-Quality Utilization, Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, Changzhou University, Changzhou 213164, PR China; State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Lifes, Hubei University, Wuhan 430062, Hubei Province, PR China.

Bioresource technology

|June 9, 2023

概括

一个新的生物工艺有效地使用工程化大肠杆菌 (E. coli) 将素衍生的素转化为利胺. 这种方法增强了从关键的生物聚合物宁中生产有价值的生物基化合物.

科学领域:

生物技术是生物技术.
绿色化学绿色化学
生物聚合物的价值化

背景情况:

氨酸是生产有价值的生物基化合物的关键生物聚合物.
瓦尼林是由素衍生而来的,是瓦尼利胺的前体,是精细化学品和制药中的关键中间体.

研究的目的:

开发一种富有成效的全细胞生物转化过程,使素变成利胺.
为了优化生物转化,使用深度优化溶剂-表面活性剂-H2O介质.

主要方法:

一种表达 ω - 转氨酶和 L - 氨酸脱酶的复合大肠杆菌30CA菌株被设计出来.
瓦尼林的生物转化是在含有深度环氧溶剂 (ChCl:LA) 和表面活性剂 (PEG-2000) 的介质中进行的.
进行了反应条件的优化,包括温度和pH值.

主要成果:

经过工程设计的大肠杆菌成功地将香草素转化为利胺.
获得的最高利胺产量是从60毫米利中获得的90.0%.
优化过程证明了在环保环境中提高生物传染效率.

结论:

建立了一种有效的生物工艺,用于将素衍生的素转化为利胺.
使用工程细菌和环保媒介显示了对素的价值化潜力.
这种方法有助于从生物质中可持续生产增值化合物.

关键词:

生物传递剂的产生.深度欧性溶剂溶剂在Lignin的基础上.瓦尼林是一种瓦尼利胺氨酸是什么

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