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Updated: Jul 27, 2025

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综合转录组和代谢组分析确定了CsERF003,一种类ERF转录因子,作为黄类激活剂

Haoliang Wan¹, Yihui Liu², Tongtong Wang²

¹College of Horticulture, Qingdao Agricultural University/Laboratory of Quality & Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs/National Technology Centre for Whole Process Quality Control of FSEN Horticultural Products (Qingdao)/Qingdao Key Lab of Modern Agriculture Quality and Safety Engineering, Qingdao 266109, China; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, China.

Plant science : an international journal of experimental plant biology

|June 9, 2023

概括

发现CsERF003,一种转录因子,增强了黄酸生产和水果颜色. 这种基因显示出提高水果质量和设计植物天然黄化合物的潜力.

科学领域:

植物科学植物科学
生物技术是生物技术.
农业科学农业科学

背景情况:

转录因子 (TF) 调制是改善植物黄酸含量的关键策略.
与野生类型 (WT) 相比,一个"Newhall"腹色突变 (MT) 呈现出降低的黄和黄水平和基因表达.

研究的目的:

为了研究下调ERFTF,CsERF003,在黄类生物合成中的作用.
评估CsERF003在提高水果质量和黄类蛋白质的潜力.

主要方法:

在类水果中过时过度表达CsERF003,以分析黄和氨酸含量.
在"微"番茄中过度表达CsERF003,以评估水果颜色和黄积累.
在烯胺和黄胺生物合成途径中的关键酶的基因表达分析.

主要成果:

过度表达CsERF003增加了中的主要黄类化合物 (naringeninchalcone,kaempferolrutinoside) 和氨酸.
番茄中CsERF003的过度表达导致了更深色,更红色的水果,具有明显更高的naringeninchalcone和kaempferolrutinoside含量.
观察到烯 (PAL,4CH,4CL) 和黄 (CHS,CHI) 生物合成基因的上调,而其他多基因 (HCT,CCR) 的下调.

结论:

CsERF003引导碳流向黄类生物合成,增强总黄类含量.
CsERF003是一种有前途的候选基因,可以改善水果质量,并在作物中改造黄类蛋白质.

关键词:

类黄类黄类化合物这是一种flavonol糖化物.工厂工程工厂工程工厂工程聚甲化聚甲化黄.转录因子是一种转录因子.

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