人类植入前胚胎的血统分离是YAP1和TEAD1所规定的
Marius Regin1, Wafaa Essahib2, Andrej Demtschenko1
1Research Group Reproduction and Genetics (REGE), Vrije Universiteit Brussel, Brussels, Belgium.
Human reproduction (Oxford, England)
|June 9, 2023
概括
人类胚胎发育涉及两个关键的血统分离事件. 在这些早期阶段,TEAD1和YAP1信号传递在明确体 (TE) 和原始内皮 (PrE) 细胞命运方面发挥着至关重要的作用.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 人类植入前发展涉及关键的血统分离事件.
- 转录因子如TEAD1和YAP1在这些早期分离中的作用尚未完全理解.
- 以前的小鼠研究表明,YAP1/TEAD信号参与了早期胚胎血统的建立.
研究的目的:
- 调查在人类植入前发育过程中参与第一和第二个血统分离事件的过程和转录因子.
- 确定TEAD1和YAP1信号传递在特异性体 (TE),表皮质 (EPI) 和原始内皮 (PrE) 细胞中的作用.
主要方法:
- 188个人类植入前胚胎 (受精后4-6天) 的发育时间表.
- 对于关键的血统标记 (GATA3,NANOG,GATA4,SOX17) 和Hippo通路组件 (YAP1,TEAD1,TEAD4) 的胚胎被固定和免疫.
- 对焦成像和分析共定位模式和蛋白质的核丰富.
主要成果:
- 压缩是一个异质的过程,在压缩结束时 (C2阶段) 建立了内部和外部细胞.
- YAP1主要存在于极化外部/TE细胞中,而在非极化内部/ICM细胞中则不存在.
- TEAD1和YAP1在前体TE细胞以及原始内皮 (PrE) 细胞中共定位,这表明它们参与了这两种血统分离.
结论:
- 在人类胚胎中,TEAD1和YAP1信号与第一个 (TE规范) 和第二个 (EPI/PrE规范) 血统分离事件有关.
- 体 (TE) 差异化可以独立于细胞极性开始.
- 这项研究为进一步对早期人类胚胎发生的功能性研究提供了路线图.
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