水素在生长和发育中的作用,调节,以及Ganoderma lucidum的非生物应激抵抗力
Jinjin Qiao1, He Liu1, Peng Xue1
1Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Weigang Road, Nanjing 210095, Jiangsu, P.R. China.
FEMS microbiology letters
|June 9, 2023
概括
水蛋白1 (Hyd1) 对于甘光的发育和非生物应激耐药性至关重要. 调节器AreA负面控制hyd1表达,影响的形成和生存.
科学领域:
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
背景情况:
- 菌水蛋白对生理功能至关重要,包括疏水性,毒性,生长和发育.
- 在Ganoderma lucidum中,水蛋白的分子调节在很大程度上仍然没有被描述.
研究的目的:
- 调查水蛋白1 (Hyd1) 在Ganoderma lucidum发育和应激反应中的作用.
- 阐明控制hyd1基因表达的调节机制,特别是与代谢的关系.
主要方法:
- 在不同发育阶段对hyd1的基因表达分析.
- 构建和分析水1静音菌株.
- 电泳运动转移试验 (EMSA) 测定转录因子结合.
- 在各种源和非生物压力条件下评估水1表达.
主要成果:
- 在G. lucidum中,hyd1基因对于原始形成至关重要;沉默hyd1阻止了启动.
- 转录因子AreA负面调节hyd1表达,与其促进体结合.
- 在酸盐中,hyd1的表达显著高于氨的源,对于抗热,细胞壁和盐的应力至关重要.
结论:
- 1在G. lucidum的发育和适应非生物压力的过程中发挥着关键作用.
- AreA是hyd1的关键负调节剂,将代谢与水表达在更高的基菌体中联系起来.
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