乳植物菌植物:一个新的包容体生产细菌的例子
Ricardo Baltà-Foix1, Caterina Serrano-Adrover1, Adrià López-Cano1
1Programa de Producció de Remugants, Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Caldes de Montbui, 08140, Spain.
Microbial cell factories
|June 9, 2023
概括
无脂多糖体 (LPS) 的细菌Lactiplantibacillus plantarum在重组生产过程中形成蛋白质聚合物 (含体). 这些聚合物含有活性蛋白质,在生物制药行业中具有潜在的应用.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 蛋白质工程是指蛋白质工程.
背景情况:
- 乳酸细菌,包括Lactiplantibacillus plantarum,越来越多地被用作重组细胞工厂.
- 虽然以前认为它不会聚合,但Lactococcus lactis产生含有活性蛋白质的包容体 (IB).
- 在L. plantarum中重组蛋白质的聚合以前没有被描述.
研究的目的:
- 为了研究L. plantarum中蛋白质聚合物的形成.
- 描述这些聚合物并评估它们的潜在应用.
主要方法:
- 在L. plantarum中,牛类金属蛋白酶9 (MMP-9cat) 的催化域的重组表达,这是一个容易聚集的蛋白质.
- 电子显微镜用于可视化蛋白质聚合物.
- 分离聚合物的净化和生物化学分析.
主要成果:
- 电子显微镜证实了L. plantarum中电子密集的包容体 (IB) 的存在.
- 孤立的IBs是光滑的,圆的,尺寸为250-300nm.
- IBs中的蛋白质完全活跃,可以溶解产生活性蛋白质或作为活性纳米粒子使用.
结论:
- 在重组蛋白质生产条件下,Lactiplantibacillus plantarum形成了包括体.
- 这些聚合物具有类似于其他表达系统 (如大肠杆菌和乳糖菌) 中发现的特性.
- 植物是一种可行的,没有LPS的替代品,可以通过包含体生产生物制药蛋白质.
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