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塔乌罗斯氧胆酸通过EGFR/p-Akt/CREB1通路在介酶体干细胞中增强骨质分化

Hyojin Kang¹, Sunsik Yang², Jun Lee¹

¹Department of Oral and Maxillofacial Surgery, College of Dentistry, Wonkwang University, 77 Dunsan-ro, Seo-gu, Daejeon 35233, Republic of Korea.

|June 10, 2023

概括

塔乌尔苏氧胆酸 (TUDCA) 增强了人体介质干细胞 (hMSCs) 的骨质分化. 这个过程涉及表皮生长因子受体 (EGFR) 信号通路,促进骨再生.

科学领域:

干细胞生物学干细胞生物学
再生医学是一种再生医学.
生物化学生物化学

背景情况:

介质细胞干细胞 (MSC) 对于再生医学至关重要,特别是在骨修复方面.
陶氏氧醇酸 (TUDCA) 越来越多地用于基于细胞的疗法.
图德卡对hMSCs骨质性分化的影响的确切机制尚未完全理解.

研究的目的:

阐明 TUDCA 诱导人类介质干细胞 (hMSC) 骨质基因分化机制.
研究表皮生长因子受体 (EGFR) 信号通路在TUDCA介导的骨质生成中的作用.

主要方法:

通过WST-1测定来评估细胞增殖.
通过性酸酶活性和阿利沙林红色染色确认了骨质分化.
量化基因表达与骨分化和信号通路相关,使用实时PCR.

主要成果:

图德卡显著增强了hMSC的扩散和骨质分化,以剂量依赖的方式.
骨质基因分化基因的升级,特别是表皮生长因子受体 (EGFR) 和cAMP响应元素结合蛋白1 (CREB1).
抑制EGFR信号传递显著减少骨质原体分化和CREB1,环林D1和环林E1.1的表达.

结论:

TUDCA促进了hMSCs的骨质分化.
EGFR/p-Akt/CREB1信号通路对于TUDCA诱导的骨质生成至关重要.
图德卡是治疗骨疾病的一种有前途的治疗剂.

关键词:

皮肤上生长因子受体.人类中介细胞干细胞骨质性分化的骨质性差异化塔乌尔索多氧胆酸是什么意思

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