基斯脱乙酶GiSRT2 负面调节在体内的黄类生物合成
Jiangyi Zeng1,2,3, Yun Huang1,3, Lijun Zhou1,3
1Guangdong Provincial Key Laboratory of Applied Botany & Guangdong Provincial Key Laboratory of Digital Botanical Garden, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Cells
|June 10, 2023
概括
尼古丁胺胺 (NIC) 增强了药用甘中的利可卡龙A (LCA) 积累. 一种组织素脱乙酶,GiSRT2,负面调节LCA生物合成,而GiLMT1对其产生至关重要.
科学领域:
- 植物生物化学 植物生物化学
- 药用植物的研究研究.
- 分子生物学分子生物学
背景情况:
- 甘花 inflata Batalin是一种有价值的药用甘物种.
- 利科哈尔科恩A (LCA),一个关键的黄类,积聚在G. inflata的根.
- 生物合成途径和LCA积累的调节在很大程度上是未知的.
研究的目的:
- 为了研究Licochalcone A (LCA) 在Glycyrrhiza inflata中的积累调节.
- 识别参与LCA生物合成及其调节网络的基因.
主要方法:
- 治疗G. inflata幼苗的治疗方法是用一个组织脱乙酶 (HDAC) 抑制剂,尼古丁胺胺 (NIC).
- 在转基因毛发根中使用RNA干扰 (RNAi) 和过度表达 (OE) 的GiSRT2的功能分析.
- 在RNAi-GiSRT2系中对转录组和代谢组的联合分析.
- 基因表达分析和GiLMT1.1.的功能验证.
主要成果:
- NIC治疗增强了LCA和总黄积累.
- 通过RNAi介导的GiSRT2沉默增加了LCA和总类蛋白水平,表明它具有负面的调节作用.
- 一种O-甲基转移酶GiLMT1在RNAi-GiSRT2系中被上调,并被证明对LCA生物合成至关重要.
- GiSRT2 在黄类生物合成途径中起负调节作用.
结论:
- 在G. inflata. 中,GiSRT2在调节黄类生物合成方面发挥着至关重要的作用.
- GiLMT1被确定为Licochalcone A生物合成途径中的一个关键基因.
- 这项研究为合成生物学方法提供了洞察力,以增强LCA生产.
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