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在条件下生成肥胖糖尿病小鼠模型
Tiago Bordeira Gaspar1,2,3,4, Tito Teles Jesus1,2, Maria Teresa Azevedo1,2
1Instituto de Investigação e Inovação em Saúde (i3S), University of Porto, 4200-135 Porto, Portugal.
Cancers
|June 10, 2023
概括
失去Atrx会导致内分泌功能障碍和肥胖,但不会导致胰腺神经内分泌瘤. 这种肥胖的糖尿病小鼠模型对代谢和癌症研究有价值.
科学领域:
- 内分泌学 在内分泌学.
- 遗传学 遗传学 是一个
- 在瘤学瘤学.
背景情况:
- 丢失Atrx (阿尔法血症/精神衰退综合征X链接) 不足以引起胰腺神经内分泌瘤 (PanNETs).
- Atrx在内分泌功能障碍中起着重要作用.
研究的目的:
- 描述Pdx1-Cre;AtrxKO (P.AtrxKO) 基因工程小鼠模型 (GEMM) 对于PanNET形成和内分泌干扰.
- 研究Atrx在代谢平衡和胰腺健康中的作用.
主要方法:
- 使用了Pdx1-Cre;AtrxKO GEMM,将淘汰赛 (P.AtrxHOM) 与野生类型 (P.AtrxWT) 的 littermates进行比较.
- 在24个月内监测代谢参数,包括体重,高血糖和葡萄糖不耐受.
- 对胰腺和肝脏进行了基因病理分析,以检测脂肪透和脂肪.
主要成果:
- 在P.AtrxKO小鼠中没有开发的PanNETs.
- 雄性和雌性P.AtrxHOM小鼠均表现出超重/肥胖,高血糖和葡萄糖不耐受.
- 在P.AtrxHOM小鼠中观察到胰腺和周胰腺脂肪透和肝脏宏观肌肉肥胖症的显著增加.
结论:
- 在P.AtrxKO小鼠中Atrx缺乏导致肥胖和糖尿病,但不是PanNETs.
- 这种GEMM是研究代谢障碍的宝贵工具,并可能用于调查额外的致癌事件.
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