通过对33种野生菌株的形态分子表征来识别8种高产菌株
Manoj Nath1, Anupam Barh1,2, Annu Sharma3
1ICAR-Directorate of Mushroom Research, Solan 173 213, India.
Foods (Basel, Switzerland)
|June 10, 2023
概括
研究人员通过形态和分子特征鉴定确定了八种高产的乳 (Calocybe indica) 菌株. 这一发现提高了热带和亚热带地区的种植潜力,帮助育种者和种植者将这种食用商业化.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物育种 植物育种
- 生物技术是生物技术.
背景情况:
- Calocybe indica或乳是一种热带和亚热带种植的受欢迎的食用物种.
- 高产品种的有限可用性阻碍了其更广泛的采用和商业成功.
研究的目的:
- 描述来自印度不同地区的Calocybe indica生殖质.
- 识别优质品种,以提高产量和质量属性.
- 分析遗传多样性以了解菌株关系.
主要方法:
- 33种C. indica菌株的形态,农学和分子 (ITS测序,SRAP标记) 特性.
- 用PCR放大,测序和核酸分析进行菌株鉴定.
- 根据基因相似性,UPGMA对基因分析进行了基因分析,以组分菌株.
主要成果:
- 所有菌株的确认身份为Calocybe indica.
- 鉴定出八种高产品种比对照品种优越 (DMRO-302).
- 通过UPGMA分析,SRAP标记揭示了遗传多样性,将菌株分类为三个群.
- DMRO-54显示出高抗氧化剂和含量;DMRO-202和DMRO-299显示出最大的蛋白质含量.
结论:
- 该研究成功地确定了具有理想的营养和生化特征的高产Calocybe indica菌株.
- 遗传多样性分析为选择性育种计划提供了基础.
- 这些发现将促进乳的商业化和更广泛的种植.
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