硫酸多糖的结构特征,在体外抗氧化和抗瘤活动的硫酸多糖从
Pin Gong1, Mengrao Wang1, Yuxi Guo1
1School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
Foods (Basel, Switzerland)
|June 10, 2023
概括
一种来自Siraitia grosvenorii的新型硫酸多糖,S-SGP,显示出显著的抗氧化和抗癌特性. 这种化合物有效地清除自由基,并在体外抑制各种人类癌细胞的生长.
科学领域:
- 自然产品化学 自然产品化学
- 生物化学 生物化学
- 药理学 药理学 是一个学科.
背景情况:
- 西拉提亚大黄产生一种天然的葡萄糖多糖,SGP-1.
- SGP-1 呈现出由链接的葡萄糖单元组成的复杂结构.
研究的目的:
- 合成和描述SGP-1的硫酸衍生物,命名为S-SGP.
- 在体外评估S-SGP的抗氧化和抗癌活性.
主要方法:
- 使用硫酸方法硫化SGP-1.
- 鉴定包括FT-IR,GPC和SEM分析.
- 进行了体外抗氧化剂测试 (DPPH,氧化,超氧化基清除) 和针对HepG2,MDA-MB-231和A549癌细胞系的细胞毒性测试.
主要成果:
- 硫酸衍生物S-SGP的替代度为0.62和分子量为1.34 × 104 Da.
- S-SGP表现出强大的自由基清除活性,随着度的增加而增加.
- S-SGP 抑制肝瘤,乳腺和肺癌细胞的增殖.
- 在A549细胞中,S-SGP降低了线粒体膜潜力,诱导了亡,并改变了与亡相关的基因和蛋白质表达.
结论:
- 硫酸多糖体S-SGP具有显著的抗氧化和抗癌特性.
- 通过诱导亡,S-SGP显示出作为癌症治疗的治疗剂的潜力.
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