通过使用不同类型的酶在体内制备的酶化化缩粉的多尺度结构洞察力
Namei Ren1, Xinzhong Hu1, Zhen Ma1
1College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China.
Foods (Basel, Switzerland)
|June 10, 2023
概括
使用胰腺素 (PC-EHSC) 和β-氨酸酶 (βA-EHSC) 的豆粉缩物的酶性水解产生了具有同质葡萄糖链的更有序结构. 热稳性α-氨酶 (HS-EHSC) 导致晶度降低,影响了粉结构组织.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
背景情况:
- 豆粉缩剂 (CCLSC) 是复杂的生物聚合物,具有潜在的应用.
- 了解由酶解水解引起的结构变化对于定制它们的特性至关重要.
研究的目的:
- 研究各种酶对豆粉水解物的多尺度结构特征的影响.
- 为了比较从胰腺素 (PC-EHSC),热稳定性α-氨酶 (HS-EHSC),β-氨酶 (βA-EHSC),氨糖酶 (AMG-EHSC) 和多酶 (βA-HS-AMG-EHSC) 获得的产品的结构特征.
主要方法:
- 豆粉缩物的酶性水解.
- 形态分析. 形态分析.
- 里埃变换红外光谱法 (FTIR). 福利埃变换红外光谱法.
- 固态13C CP/MAS NMR光谱法. 固态13C CP/MAS NMR光谱法. 固态13C CP/MAS NMR光谱法. 固态13C CP/MAS NMR光谱法. 固态13C CP/MAS NMR光谱法.
- 在X射线衍射 (XRD).
- 微角X射线散射 (SAXS) 是一种微角X射线散射技术.
- 确定多分散性指数 (DPn).
主要成果:
- FTIR和NMR表明粉素-蛋白质-脂质复合物的潜在形成.
- XRD显示了PC-EHSC和βA-EHSC的突出V型衍射峰值,与较低的DPn相关.
- PC-EHSC表现出最高的结晶度和最低的DPn,这表明同质的葡萄糖链.
- 在HS-EHSC中,相对晶度最低,表明结构排序不佳.
- 与CCLSC相比,SAXS显示PC-EHSC和βA-EHSC的散射强度增加.
结论:
- 酶性水解显著改变了豆粉缩物的多尺度结构.
- 胰腺素和β-氨基酶治疗促进了有序粉结构的形成,具有均的分子量分布.
- 热稳定的α-氨酶在创建有序粉结构方面效率较低.
- 结果提供了关于根据特定生理性质量调整粉水解盐的见解.
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