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Updated: Jul 27, 2025
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Synthesis of an Intein-mediated Artificial Protein Hydrogel
Published on: January 27, 2014
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的酶性水解
Legesse Shiferaw Chewaka1, Chan Soon Park1, Youn-Soo Cha2
1Department of Agro-Food Resources, National Institute of Agricultural Science, Rural Development Administration, Wanju 55365, Republic of Korea.
Foods (Basel, Switzerland)
|June 10, 2023
概括
努鲁克提取物缩剂 (NEC) 有效地解粉 (MW) 蛋白质,与商业酶相比,产生更优越的营养和功能性质. 这种天然的替代品显示出对成本效益高的蛋白质增强有希望.
科学领域:
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
- 酶学 是一种酶学.
背景情况:
- 酶性蛋白质水解提高了饮食蛋白质质量,因而越来越需要天然的酶来源.
- 可食用的昆虫,如粉虫 (Tenebrio molitor),是可持续的蛋白质来源.
- 努鲁克提取物缩物 (NEC) 是传统的发酵起始剂,富含酶.
研究的目的:
- 使用NEC生产粉虫 (MW) 蛋白质水解剂.
- 为了比较由NEC生产的MW水解盐的营养,功能和感觉特性,与商业蛋白质酶 (酶和风味酶) 的营养,功能和感觉特性.
- 评估NEC作为天然替代商业蛋白质酶用于昆虫蛋白质水解的潜力.
主要方法:
- 使用努鲁克提取物缩物 (NEC) 进行脱脂粉 (MW) 的水解.
- 测量了原始努鲁克提取物 (CNE),NEC,酶和风味酶的蛋白酶活性.
- 分析了水解剂的营养 (自由氨基酸),功能 (抗氧化剂和 ACE 抑制活性) 和感官特性.
主要成果:
- NEC表现出高的蛋白酶活性 (12.71单位/毫升).
- 使用NEC的MW水解产生了15.10%的水解度和35.92%的产量.
- 与商业酶化剂相比,NEC生产的化剂的自由氨基酸含量 (90.37 mg/g) 显著更高,抗氧化和 ACE 抑制活性更强.
- 感官特性,包括味,甜味和味,都得到了改善.
结论:
- 努鲁克提取物缩物 (NEC) 是一种有效的天然酶来源,用于粉 (MW) 蛋白质水解.
- 来自NEC的MW水解剂提供了优越的营养,功能和感官益处,而不是商业蛋白质酶衍生水解剂.
- 在昆虫蛋白质加工中,NEC为商业蛋白酶提供了一个可行的,潜在的成本效益高的替代方案.
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