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相关概念视频

Hydrolysis01:15

Hydrolysis

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Overview
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
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Esters to Carboxylic Acids: Acid-Catalyzed Hydrolysis

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Hydrolysis of esters under acidic conditions proceeds through a nucleophilic acyl substitution. In the presence of excess water, the reaction proceeds in a reversible manner, forming carboxylic acids and alcohols.
During hydrolysis, the ester is first activated towards nucleophilic attack through the protonation of the carboxyl oxygen atom by the acid catalyst. The protonation makes the ester carbonyl carbon more electrophilic. In the next step, water acts as a nucleophile and adds to the...
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Phase I Reactions: Hydrolytic Reactions01:15

Phase I Reactions: Hydrolytic Reactions

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Hydrolysis, a cornerstone of phase I biotransformation reactions, uses water to cleave chemical bonds. This process is pivotal in drug metabolism, generating more polar metabolites that can be easily excreted.
An important hydrolytic reaction is ester hydrolysis. Ester bonds, often found in prodrugs, are broken down, increasing the solubility of drugs like aspirin and lidocaine for more straightforward elimination. Amide hydrolysis is another critical reaction, targeting amide bonds prevalent...
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Amides to Carboxylic Acids: Hydrolysis01:28

Amides to Carboxylic Acids: Hydrolysis

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Amides can undergo either acid-catalyzed hydrolysis or base-promoted hydrolysis through a typical nucleophilic acyl substitution. Each hydrolysis requires severe conditions.
Acid-catalyzed hydrolysis:
Hydrolysis of amides under acidic conditions yields carboxylic acids. Since the reaction occurs slowly, hydrolysis requires the conditions of heat.
The mechanism begins with the protonation of the carbonyl oxygen by the acid catalyst. The protonation makes the amide carbonyl carbon more...
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Lysosomal Hydrolases01:22

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Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
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Enzymes02:34

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Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
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相关实验视频

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Synthesis of an Intein-mediated Artificial Protein Hydrogel
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Synthesis of an Intein-mediated Artificial Protein Hydrogel

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的酶性水解.

Legesse Shiferaw Chewaka1, Chan Soon Park1, Youn-Soo Cha2

  • 1Department of Agro-Food Resources, National Institute of Agricultural Science, Rural Development Administration, Wanju 55365, Republic of Korea.

Foods (Basel, Switzerland)
|June 10, 2023
PubMed
概括
此摘要是机器生成的。

努鲁克提取物缩剂 (NEC) 有效地解粉 (MW) 蛋白质,与商业酶相比,产生更优越的营养和功能性质. 这种天然的替代品显示出对成本效益高的蛋白质增强有希望.

关键词:
布里奥·莫莱托尔 (Tenebrio molitor) 是一个布里奥的动物.阿尔卡莱斯 (alcalase) 是一种芳香酶是一种芳香酶.努鲁克 (nuruk) 是一个名为"努鲁克" (nuruk) 的城市.蛋白质酶蛋白质酶是一种蛋白质.蛋白质解蛋白质解蛋白质解蛋白质

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科学领域:

  • 食品科学 食品科学 食品科学
  • 生物技术是生物技术.
  • 酶学 是一种酶学.

背景情况:

  • 酶性蛋白质水解提高了饮食蛋白质质量,因而越来越需要天然的酶来源.
  • 可食用的昆虫,如粉虫 (Tenebrio molitor),是可持续的蛋白质来源.
  • 努鲁克提取物缩物 (NEC) 是传统的发酵起始剂,富含酶.

研究的目的:

  • 使用NEC生产粉虫 (MW) 蛋白质水解剂.
  • 为了比较由NEC生产的MW水解盐的营养,功能和感觉特性,与商业蛋白质酶 (酶和风味酶) 的营养,功能和感觉特性.
  • 评估NEC作为天然替代商业蛋白质酶用于昆虫蛋白质水解的潜力.

主要方法:

  • 使用努鲁克提取物缩物 (NEC) 进行脱脂粉 (MW) 的水解.
  • 测量了原始努鲁克提取物 (CNE),NEC,酶和风味酶的蛋白酶活性.
  • 分析了水解剂的营养 (自由氨基酸),功能 (抗氧化剂和 ACE 抑制活性) 和感官特性.

主要成果:

  • NEC表现出高的蛋白酶活性 (12.71单位/毫升).
  • 使用NEC的MW水解产生了15.10%的水解度和35.92%的产量.
  • 与商业酶化剂相比,NEC生产的化剂的自由氨基酸含量 (90.37 mg/g) 显著更高,抗氧化和 ACE 抑制活性更强.
  • 感官特性,包括味,甜味和味,都得到了改善.

结论:

  • 努鲁克提取物缩物 (NEC) 是一种有效的天然酶来源,用于粉 (MW) 蛋白质水解.
  • 来自NEC的MW水解剂提供了优越的营养,功能和感官益处,而不是商业蛋白质酶衍生水解剂.
  • 在昆虫蛋白质加工中,NEC为商业蛋白酶提供了一个可行的,潜在的成本效益高的替代方案.