Klebsiella pneumoniae原体的表征和多样性
Fuqiang Kang1, Zili Chai1, Beiping Li1
1Laboratory of Advanced Biotechnology & State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing 100071, China.
International journal of molecular sciences
|June 10, 2023
概括
菌在Klebsiella pneumoniae基因组中广泛存在,携带毒性和耐药性基因. 它们的存在影响了菌株特征,并表明了染色体与等离子体之间不同的进化路径.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 肺炎Klebsiella是一种显著的机会性病原体,其耐药性率越来越高.
- 移动遗传元素,特别是prophages,在细菌进化和毒性中发挥着至关重要的作用.
- 了解益生菌与宿主相互作用对于打击抗生素耐药性至关重要.
研究的目的:
- 为了识别和描述Klebsiella pneumoniae基因组中的prophages.
- 为了研究K. pneumoniae.中的prophages的分布和多样性.
- 探索菌对K. pneumoniae毒性和抗生素耐药性的潜在影响.
主要方法:
- 来自NCBI数据库的1437个完全组装的K. pneumoniae基因组的生物信息分析.
- 识别和量化整合到染色体和等离子体中的prophages.
- 对菌体和基因组DNA (GC含量) 的比较分析,以推断进化起源.
主要成果:
- 在1437个K. pneumoniae基因组中确定了15946个prophages (染色体上的9755,等离子体上的6191).
- 前体是多样化和广泛分布的,编码假定毒性因素和抗生素耐药性基因.
- 与K. pneumoniae菌株类型相关的原存在和类型,以及GC含量差异表明了染色体与等离子体原的外来起源和独特的进化轨迹.
结论:
- 菌在K. pneumoniae中非常普遍,显著影响其基因组.
- 体有助于K. pneumoniae的毒性和抗生素耐药性.
- 前体特征表明它们是获得的移动元素,在染色体和质粒上具有不同的进化历史.
相关概念视频
Viral Replication: Lysogenic Cycle
87
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
87
Lysogenic Cycle of Bacteriophages
62.6K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
62.6K
DNA Bacteriophages
62
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
62
Genomic DNA in Prokaryotes
44.1K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
44.1K
Bacterial Phylum Proteobacteria
48
Proteobacteria, one of the largest and most diverse bacterial phyla, encompasses a wide range of Gram-negative bacteria distinguished by their outer membrane composed of lipopolysaccharides. These microorganisms exhibit various metabolic capabilities, including phototrophy, chemolithotrophy, and heterotrophy, and thrive in diverse environments from soil to aquatic systems and host-associated niches. The phylum is divided into six classes: Alphaproteobacteria, Betaproteobacteria,...
48
Diversity of Protists I
46
Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...
46


