集成的多态学揭示了非添加式表达的小RNA在玉米植物高度的异质化中的重要作用
Jie Zhang1,2,3, Yuxin Xie2, Hongwei Zhang2
1Center of Seed Science and Technology, Beijing Innovation Center for Seed Technology (MOA), Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
International journal of molecular sciences
|June 10, 2023
概括
小RNAs (sRNAs) 在玉米植物高度异构中发挥着关键作用. 微RNA (miRNA) 和小干扰RNA (siRNA) 的非添加表达影响生长和压力路径,揭示了新的表观遗传调节机制.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 异质化或混合活力对于作物改善至关重要,但其表观遗传基础尚未完全理解.
- 小RNAs (sRNAs),包括microRNAs (miRNAs) 和小干扰RNAs (siRNAs),是关键的表观遗传调节者.
- 对于sRNAs在调解异质化的特定作用,特别是植物的高度,需要进一步研究.
研究的目的:
- 为了研究小RNAs (sRNAs) 在玉米植物高度 (PH) 异位化中的作用.
- 分析与其父系相比,玉米杂交品种中miRNA和siRNA的非添加式表达模式.
- 通过多omics集成,阐明sRNAs在PH异构中的调节机制.
主要方法:
- 从玉米杂交物和父系线获得的多奥米克测序数据 (sRNAome,转录组,甲基组) 的综合分析.
- 识别和量化非添加式表达的miRNAs和24-nt siRNAs.
- 在sRNA表达,基因表达和DNA甲基化模式之间的相关性分析.
主要成果:
- 显著比例的miRNAs (18.61%) 和24-nt siRNAs (54.00%) 在混合体中表现出非添加式表达.
- 非添加式表达的miRNAs通过调节植物生长,生殖和应激反应途径来影响PH异构.
- 非添加性甲基化事件与非添加性表达的siRNA相关,具有与特定生物过程相关的独特siRNA表达模式 (LPE与HPE).
结论:
- 小RNAs,特别是非添加式表达的miRNAs和siRNAs,是玉米植物高度异位的关键调节者.
- sRNAs通过协调基因表达和表观遗传修饰来影响异构,跨越生长,新陈代谢和应激反应途径.
- 这项研究为植物中sRNA介导的异质变异的表观遗传机制提供了新的见解.
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