帕特诺利德在淋巴细胞恶性瘤中诱导ROS介导的亡
Joana Jorge1,2,3,4, Joana Neves1, Raquel Alves1,2,3,4
1Laboratory of Oncobiology and Hematology (LOH), University Clinic of Hematology, Faculty of Medicine (FMUC), University of Coimbra, 3000-548 Coimbra, Portugal.
International journal of molecular sciences
|June 10, 2023
概括
帕瑟诺利德是一种NF-κB抑制剂,有效降低了新陈代谢活动,并在淋巴细胞恶性瘤中诱导细胞亡. 这种天然化合物显示出作为B细胞和T细胞癌症的新型治疗剂的前景.
科学领域:
- 在瘤学瘤学.
- 药理学 药理学是指药理学的学科.
- 分子生物学分子生物学
背景情况:
- 淋巴瘤恶性瘤是各种癌症,通常是由构成性核因子kappa B (NF-κB) 信号驱动的.
- 帕特诺利德是一种天然化合物,已知可以抑制NF-κB信号传递,并用于偏头痛和关节炎.
研究的目的:
- 为了评估在各种淋巴瘤瘤中帕瑟诺利德的体外疗效.
- 调查帕瑟诺利德对癌细胞影响的机制.
主要方法:
- 在多个淋巴细胞细胞系 (MM,DLBCL,BL,B-ALL,T-ALL) 中使用resazurin测定对帕瑟诺利德对代谢活动的影响进行评估.
- 评估细胞死亡,细胞循环,线粒体膜潜力 (ΔΨmit),活性氧物种 (ROS),降低型谷氨 (GSH) 和NF-κB p65酸化通过流动细胞计.
- 使用qPCR对CMYC,TP53,GPX1和TXRND1的基因表达量化.
主要成果:
- 帕瑟诺利德降低了所有测试的淋巴状细胞系中的代谢活性,时间,剂量和细胞系依赖.
- 帕瑟诺利德通过细胞亡诱导细胞死亡,其特征是ROS增加,GSH减少,并在所有细胞系中减少ΔΨ.
- 发现特定的作用机制是细胞系依赖的.
结论:
- 帕瑟诺利德在体外对B型和T型淋巴细胞恶性瘤具有显著的疗效.
- 帕瑟诺利德通过ROS生成和线粒体潜力的破坏诱导亡.
- 帕瑟诺利德值得进一步研究,因为它是淋巴细胞癌症的潜在治疗剂.
关键词:
在 NF-κBB 中.作为NF-κB抑制剂的使用.急性淋巴细胞白血病急性淋巴细胞白血病灭症 (apoptosis) 是一种死亡的过程.淋巴瘤淋巴瘤是什么多发性骨髓瘤是多发性骨髓瘤的一种.氧化应激是一种氧化应激.更多相关视频
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