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欧巴丁是一种黄胺,可以抑制冠状病毒3CL蛋白酶和复制

Yea-In Park¹, Jang Hoon Kim², Siyun Lee¹

¹Division of Biological Science and Technology, Yonsei University, Wonju 26493, Republic of Korea.

International journal of molecular sciences

|June 10, 2023

概括

来自Inula japonica花的欧巴丁通过准3CL蛋白酶,有效地抑制了冠状病毒的复制. 这种天然化合物显示出作为冠状病毒感染的潜在治疗方法的希望.

科学领域:

药理学药理学是指药理学的学科.
病毒学病毒学
自然产品化学化学

背景情况:

COVID-19 疫情凸显了对新出现的冠状病毒有效治疗的迫切需要.
由于持续的病毒变体演变,现有的疫苗需要补充的抗病毒策略.

研究的目的:

为了研究eupatin的抗病毒性质,eupatin是一种从Inula japonica中分离出来的化合物.
确定欧巴丁是否抑制冠状病毒复制及其作用机制.

主要方法:

欧巴丁从Inula japonica中分离出来的方法.
在体外测试以评估SARS-CoV-2 3CL蛋白酶的抑制.
计算建模用于预测欧巴与蛋白酶的相互作用.
使用人体冠状病毒OC43 (HCoV-OC43) 进行病毒斑块测定和病毒RNA和蛋白质水平测量.

主要成果:

欧巴丁是从Inula japonica的花中分离出来的.
欧巴丁证明了SARS-CoV-2 3CL蛋白酶的抑制,计算建模支持与关键残留物的相互作用.
用eupatin治疗减少了HCoV-OC43感染细胞中的病毒斑块形成.
欧巴治疗导致病毒蛋白和RNA水平降低.

结论:

欧巴对冠状病毒表现出抗病毒活性.
欧巴抑制了冠状病毒的复制,可能是通过与3CL蛋白酶的相互作用.
日本虫可能是新型抗病毒化合物的来源.

关键词:

3CL 蛋白酶是一种3CL蛋白酶.在日本,它被称为Inula japonica.这就是SARS-CoV-2病毒.这种类型的黄类药物是flavonoid.

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