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米OsMRG702及其合作伙伴OsMRGBP通过H4乙化控制开花时间

Feng Gong¹, Kaixin Zhang¹, Jing Wen¹

¹College of Life Sciences, Nanjing Agriculture University, Nanjing 210014, China.

International journal of molecular sciences

|June 10, 2023

概括

米的开花时间由MORF相关的GENE702 (OsMRG702) 和OsMRGBP进行调节. 这些蛋白质相互作用并影响H4K5在开花基因中的乙化,影响转录和植物发育.

科学领域:

植物分子生物学植物分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
大米的遗传学大米的遗传学

背景情况:

众所周知,与MORF相关的GENE702 (OsMRG702) 能够调节大米中的花期基因.
OsMRG702的精确转录控制机制在很大程度上仍未被阐明.

研究的目的:

为了研究OsMRG702和OsMRGBP之间的相互作用.
通过表观遗传修饰,阐明OsMRG702和OsMRGBP在调节开花时间基因中的作用.

主要方法:

酵母二杂交试验以确认蛋白质与蛋白质的相互作用.
对CRISPR/Cas9淘汰突变体 (Osmrg702和Osmrgbp) 的开花时间的分析.
染色体免疫沉 (ChIP) 试验评估蛋白质结合和素乙化 (H4K5ac) 在目标基因位置.

主要成果:

OsMRG702 直接与 OsMRGBP 相互作用.
无论是Osmrg702还是Osmrgbp突变,都表现出延迟的开花和减少了关键基因如Ehd1和RFT1.1的转录.
OsMRG702 和 OsMRGBP 在 Ehd1 和 RFT1 位置合作促进 H4K5 乙化.
OsMRG702还负面调节Ghd7转录,可能通过H4K5乙化调节.

结论:

OsMRG702和OsMRGBP通过调节H4K5乙化,在调节水开花时间方面发挥着至关重要的作用.
它们既合作通过H4K5乙化增强转录,又独立通过其他机制抑制转录.

关键词:

在H4K5ac中使用.这就是OsMRG7022.我们的OsMRGBP 花开的时候,花开的时候.米米饭米饭米饭米饭.

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