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Updated: Jul 27, 2025

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
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RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

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一个优化/扩展的即时准备协议,用于以良好的产量和低成本提取细菌产生的dSRNA

Lucas Henrique Figueiredo Prates1, Maximilian Merlau1, Johanna Rühl-Teichner1

  • 1Department of Insect Biotechnology in Plant Protection, Justus Liebig University Giessen, 35394 Giessen, Germany.

International journal of molecular sciences
|June 10, 2023
PubMed
概括

我们优化了一种具有成本效益的方法来提取细菌产生的双链RNA (dsRNA). 这种改进的协议产生了高质量的dsRNA,这对于开发使用RNA干扰 (RNAi) 的可持续害虫控制解决方案至关重要.

关键词:
这是一种RNA干扰.基于RNA的生物农药基于RNAi的害虫防治方法细菌生产系统的细菌生产系统.这是一种双链RNA.dsRNA 的生产成本.液体液体提取 提取方法有机净化 有机净化有选择的提取.

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Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR11:37

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DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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