相关实验视频

Updated: Aug 16, 2026

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

前列腺癌的转录组分析,考虑风险组和TMPRSS2-ERG分子亚型

Anastasiya A Kobelyatskaya¹, Elena A Pudova¹, Irina V Katunina¹

¹Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.

International journal of molecular sciences

|June 10, 2023

概括

前列腺癌 (PCa) 复发与分子差异有关. 这项研究确定了EEF1A1P5和LPL等关键基因,为PCa患者提供了新的预后标志物.

科学领域:

在瘤学瘤学.
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

前列腺癌 (PCa) 的分子异质性有助于手术后复发的风险变化.
了解转录组概况对于识别预后标志物和治疗点至关重要.

研究的目的:

分析局部和局部发达PCa.的转录组概况.
确定具有PCa复发和预后预测潜力的新型基因.

主要方法:

101名俄罗斯PCa患者样本的RNA-Seq分析 (58个局部,43个局部高级).
生物信息学分析检查转录组特征和受影响的生物过程.
对已识别的预后标志物进行定量实时PCR (qPCR) 验证.

主要成果:

确定了EEF1A1P5,RPLP0P6,ZNF483,CIBAR1,HECTD2,OGN和CLIC4基因,这些基因在高风险PCa中具有很高的预测潜力.
发现LPL,MYC和TWIST1是中等风险PCa (格里森评分7) 的有希望的预后标志物.
通过qPCR验证证实LPL,MYC和TWIST1的统计学意义.

结论:

转录基因组分析揭示了与PCa风险和复发相关的独特分子特征.
鉴定的基因为改善PCa患者分层和管理提供了作为新生物标志物的潜力.
对这些基因的进一步研究可能会发现前列腺癌的新治疗点.

关键词:

这就是ISUP.在RNA-Seqq.这就是TMPRSS2-ERGG.基因表达的基因表达方式异质性的异质性分子亚型分子亚型路径路径路径前列腺癌是前列腺癌.风险组风险组风险组转录组 (transcriptome) 是一个转录组.

更多相关视频

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer

Published on: November 2, 2013

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

相关概念视频

Tumor Progression

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...