潜在的分子机制 潜在的减少的抗菌活动 质胺类蛋白质 暴露后的 Mytilus galloprovincialis 和
Carmela Marinaro1, Gennaro Lettieri1, Mariavittoria Verrillo2
1Department of Biology, University of Naples Federico II, 80126 Napoli, Italy.
International journal of molecular sciences
|June 10, 2023
概括
暴露于和的海贝在它们的原胺类 (PL) 蛋白中显示出降低了抗菌活性. 这些发现凸显了重金属污染对天然抗菌化合物的潜在影响.
科学领域:
- 海洋生物学 海洋生物学
- 生物化学 生物化学
- 环境毒理学环境毒理学
背景情况:
- 天然生物活性化合物是新型抗微生物药物的有希望的来源.
- 像鱼这样的海洋生物是这些化合物的潜在来源.
- 重金属可以改变蛋白质的结构和功能.
研究的目的:
- 为了研究 (VI) 和 (HgCl2) 暴露对Mytilus galloprovincialis.PL类蛋白质抗菌活性的影响.
- 分析重金属暴露引起的PL蛋白的结构变化.
- 为了确定受影响的PL蛋白的最小抑制度 (MIC) 和最小杀菌度 (MBC).
主要方法:
- 贝类 (Mytilus galloprovincialis) 暴露于 (VI) 和 (HgCl2) 的亚毒剂量.
- 使用电泳分析 (AU-PAGE和SDS-PAGE) 来评估PL蛋白模式的变化.
- 抗菌活性是通过MIC和MBC测定对格兰阳性和格兰阴性细菌确定的.
- 光谱学证实了PL蛋白的构造变化.
主要成果:
- 暴露于高剂量的和显著降低了PL蛋白的抗菌活性.
- 在高金属度下观察到PL蛋白的形状变化,通过电泳和光得到证实.
- 随着金属暴露的增加,观察到抗菌疗效的剂量依赖性下降.
结论:
- 重金属污染可能会损害海洋生物活性化合物的天然抗菌特性,如鱼PL蛋白.
- PL蛋白质的结构变化与它们降低的抗微生物活性有关.
- 这项研究提供了初步证据,证明重金属对海洋衍生蛋白质抗菌潜力的影响.
关键词:
梅虫 (Mytilus galloprovincialis) 是一个省份的植物.它具有抗菌活性,具有抗菌活性.光度测量 光度测量格拉姆负的阴性格拉姆阳性的阳性重金属重金属的使用.自然分子是天然的分子.蛋白质胺类蛋白质类似的蛋白质.精子蛋白质 精子蛋白质更多相关视频
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