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不同类型的重组SARS-CoV-2尖端蛋白对循环单细胞结构的影响

Marco Vettori¹, Francesco Dima¹, Brandon Michael Henry²

¹Section of Clinical Biochemistry, University of Verona, 37129 Verona, Italy.

International journal of molecular sciences

|June 10, 2023

概括

来自祖先,阿尔法和三角形变体的SARS-CoV-2尖端蛋白质诱导了单细胞的显著形态变化,与Omicron变体不同. 这些变化包括增加细胞复杂性和体积异质性,表明对免疫细胞的生物学影响.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
病毒学病毒学

背景情况:

SARS-CoV-2 病毒,特别是其尖端蛋白,已与各种生物效应有关.
了解不同SARS-CoV-2变种对免疫细胞的细胞影响对于理解COVID-19的病原体至关重要.

研究的目的:

研究不同变异的复合SARS-CoV-2尖端蛋白对循环人类单细胞的生物学影响.
为了比较单细胞对来自祖先,阿尔法,三角形和Omicron变体的尖端蛋白的反应.

主要方法:

来自健康捐赠者的全人血液与不同度的重组尖端蛋白 (祖先,阿尔法,三角形,Omicron) 进行了化.
使用Sysmex XN和DI-60分析仪分析了单细胞形态和细胞特征.

主要成果:

来自Ancestral,Alpha和Delta变异的重组尖端蛋白增加了单细胞细胞复杂性和体积异质性.
核酸含量在Alpha和Delta变体的尖端蛋白中显著下降.
观察到单细胞形态异常,包括形和真空化,特别是在阿尔法和三角形变体中.

结论:

SARS-CoV-2 尖端蛋白质诱导了循环单细胞中显著的形态异常.
对单细胞的生物效应依赖于变异,祖先,阿尔法和三角形变异显示出比Omicron更明显的效应.
这些发现突显了特定SARS-CoV-2变异对先天免疫细胞功能的影响.

关键词:

在 COVID-19 疫情中,这就是SARS-CoV-2病毒.一个单细胞单细胞.形态学形态学形态学尖子蛋白质是什么?

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相关概念视频

Conjugated Proteins

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...