来自葡萄藤的转录因子VvDREB2A提高了耐寒性
Lixia Hou1, Qiqi Wu1, Xiaomin Zhu1
1Key Lab of Plant Biotechnology in University of Shandong Province, College of Life Science, Qingdao Agricultural University, Qingdao 266109, China.
International journal of molecular sciences
|June 10, 2023
概括
葡萄藤VvDREB2A转录因子增强了耐寒性. 在Arabidopsis中过度表达VvDREB2A提高了生存率,并在寒冷压力下减少了氧化损伤.
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 低温对葡萄酒种植和更广泛的葡萄产业产生重大影响.
- 转录因子DREB (干反应元素结合) 在植物对非生物应激反应中起着至关重要的作用.
研究的目的:
- 为了隔离和描述来自Vitis vinifera的VvDREB2A基因.
- 为了研究VvDREB2A在应对寒冷压力的功能.
主要方法:
- 从"Zuoyouhong"葡萄树中对VvDREB2A的基因分离和测序.
- 烟草叶子中的亚细胞局部化和酵母中的转录活动测定.
- 在不同的葡萄树组织和各种压力治疗下进行表达分析.
- 在冷应力下生成和分析VvDREB2A过度表达的Arabidopsis thaliana线.
主要成果:
- VvDREB2A编码了一个具有AP2域的核局部转录因子.
- VvDREB2A的表达是由冷,H2S,氧化和酸诱导的.
- 在阿拉比多普西斯中,VvDREB2A的过度表达增强了耐寒性,增加了生存率,减少了氧化损伤 (ROS,H2O2,MDA),增强了抗氧化酶活性,并增加了拉芬家族寡糖 (RFO) 含量.
- 感冒反应基因 (COR15A,COR27,COR6.6,RD29A) 的表达在转基因Arabidopsis.中被上调.
结论:
- 在植物中,VvDREB2A作为耐寒应激的积极调节者.
- 它的功能是清除活性氧物种,增加RFO积累,并诱导对寒冷反应的基因表达.
- 这种转录因子有可能改善像葡萄藤这样的作物中的耐寒性.
关键词:
这就是ROSOS ROS.葡萄酒 (Vitis vinifera) L. 葡萄酒 (Vitis vinifera) L. 葡萄酒 (Vitis vinifera) L. 葡萄酒 (Vitis vinifera) L. 葡萄酒 (Vitis vinifera) L. 葡萄酒 (L.这就是VvDREB2A.寒冷的压力是冷的压力.感冒与压力相关的基因拉菲诺糖家族的寡糖类.更多相关视频
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