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在Verticillium dahliae中,小氨酸丰富的蛋白VdSCP23操纵宿主免疫力

Jie Wang¹, Dan Wang², Xiaobin Ji²

¹College of Plant Protection, Shanxi Agricultural University, Taigu, Jinzhong 030801, China.

International journal of molecular sciences

|June 10, 2023

概括

来自Verticillium dahliae的小氨酸丰富蛋白VdSCP23抑制了植物免疫反应,包括细胞死亡和防御基因表达. 然而,VdSCP23对真菌生长或毒性并不重要.

科学领域:

植物病理学植物病理学
分子植物-微生物相互作用
菌效应生物学的生物学

背景情况:

由Verticillium dahliae引起的Verticillium wilt是一种破坏性土壤传播的真菌病,影响全球作物产量.
效应物,特别是小氨酸丰富的蛋白质 (SCP),对于Verticillium dahliae在感染期间操纵宿主免疫力至关重要.
许多由V. dahliae分泌的SCP的特定功能在很大程度上仍然没有特征.

研究的目的:

研究小氨酸丰富的蛋白质VdSCP23在Verticillium dahliae及其植物宿主之间的相互作用中的作用.
阐明VdSCP23影响植物免疫反应和真菌毒性的机制.

主要方法:

在植物试验中,使用尼科蒂亚娜 (Nicotiana benthamiana) 来评估VdSCP23对细胞亡,活性氧物种 (ROS) 爆发和防御基因表达的影响.
使用显微镜对VdSCP23的亚细胞定位研究.
针对位点的突变发生和断,以确定VDSCP23.23的关键功能域.
生成VdSCP23删除菌株,以评估其对于N. benthamiana,Gossypium hirsutum和Arabidopsis thaliana的真菌生长,发育和毒性的必要性.

主要成果:

VdSCP23显著抑制了植物免疫反应,包括细胞亡,ROS爆发,电解质泄漏和N. benthamiana的防御相关基因表达.
VdSCP23局限于植物细胞等离子体膜和细胞核,但其免疫抑制功能与核局限性无关.
VdSCP23的抑制活性依赖于N-糖化位点和整体蛋白质结构完整性,而不是氨酸残留物.
删除VdSCP23不会影响V. dahliae的菌生长,菌生产或对测试植物物种的毒性.

结论:

VdSCP23在抑制植物免疫反应方面发挥着重要作用,充当抑制宿主防御的毒性因子.
尽管VdSCP23具有免疫抑制功能,但它对于Verticillium dahliae的植物生长和病原性是不可或缺的.
这项研究强调VdSCP23是参与宿主免疫逃避的关键效应因子,为Verticillium病原体提供了洞察力.

关键词:

这种植物名为Verticillium dahlia.抑制植物免疫的方法这种蛋白质含有丰富的小氨酸蛋白.亚细胞局部化的局部化毒性毒性是一种毒性.

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