鉴定了一种新的RNA和蛋白质综合生物标记面板,与DKD中功能障碍相关:翻译影响
Alessandra Scamporrino1, Stefania Di Mauro1, Agnese Filippello1
1Department of Clinical and Experimental Medicine, Internal Medicine, Garibaldi-Nesima Hospital, University of Catania, 95122 Catania, Italy.
International journal of molecular sciences
|June 10, 2023
概括
识别早期糖尿病病 (DKD) 生物标志物至关重要. 一个结合血清线粒体RNA和蛋白质生物标志物的新型小组显著改善了DKD患者功能下降的诊断.
科学领域:
- 生物标志物和翻译医学
- 脏病学和内分泌学
- 分子诊断学 分子诊断
背景情况:
- 糖尿病病 (DKD) 显著增加末期病和心血管事件的风险.
- 早期识别DKD患者和预测功能下降需要敏感和特定的生物标志物.
- 之前的研究在DKD患者中发现了五种下调的血清线粒体RNA (MT-ATP6,MT-ATP8,MT-COX3,MT-ND1,MT-RNR1).
研究的目的:
- 在DKD中评估血清蛋白生物标记物 (TNFRI,TNFRII,KIM-1).
- 评估个人和组合的RNA和蛋白质生物标志物对DKD进展的诊断性能.
- 为了研究正常蛋白尿和微蛋白尿的DKD亚组中的生物标志物性能.
主要方法:
- 在不同功能阶段 (G1-G3) 的糖尿病患者中分析TNFRI,TNFRII和KIM-1的血清蛋白度.
- 蛋白质生物标志物与肌素的相关性分析,估计的膜过率 (eGFR) 和血液尿素 (BUN).
- 多逻辑回归分析用于评估组合RNA和蛋白质生物标志物面板的诊断性能,用于识别G3与G2DKD患者.
主要成果:
- 血清蛋白生物标志物 (TNFRI,TNFRII,KIM-1) 从G1到G3 DKD阶段逐渐升级,并与功能标志物相关联.
- 将特定RNA转录与蛋白质生物标记物 (TNFRI/KIM-1与任何RNA;TNFRII与MT-ATP8,MT-ATP6,MT-COX-3,MT-ND1) 结合起来,显著提高了区分G3和G2DKD的诊断准确性 (AUC>0.9,通常为1).
- 组合小组表现出增强的诊断性能,特别是在正常白蛋白和微白蛋白子组.
结论:
- 一个新的多种生物标记面板,整合血清线粒体RNA和蛋白质生物标记,显示出早期DKD诊断和预测功能下降的特殊前景.
- 与单个生物标志物相比,这种综合方法显著提高了诊断性能.
- 这些发现支持了这个多种小组的临床实用性,用于管理DKD患者并预防不良结局.
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