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全基因组识别和比较转录组方法揭示 FaMDHAR50 调节酸再生和草果的质量形成

Guoyan Hou¹, Min Yang¹, Caixia He¹

¹College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China.

International journal of molecular sciences

|June 10, 2023

概括

一个关键的基因,FaMDHAR50,被确定可以促进草中的 Askorbic 酸 (AsA) 再生. 过度表达这种基因会显著增加AsA含量,并影响水果的质量特征.

科学领域:

植物分子生物学植物分子生物学
果实生物化学果实生物化学
农业科学农业科学

背景情况:

酸 (AsA) 是草中重要的抗氧化剂,但调节其新陈代谢的基因尚不清楚.
关于识别和功能验证草ASA代谢中的关键基因的研究有限.

研究的目的:

识别和功能验证涉及草果中甲酸 (AsA) 代谢的关键基因.
研究 FaMDHAR 基因家族在 AsA 再生中的作用及其对水果质量的影响.

主要方法:

在草中全基因组识别和分析FAMDHAR基因家族.
野生类型和高AsA突变草之间的比较转录组分析.
在草果中发现的关键基因 (FaMDHAR50) 的过渡性过度表达.

主要成果:

FaMDHAR基因家族包括168个基因,大多数产物定位在叶绿体和细胞质中.
确定了FaMDHAR50作为一个关键基因,积极调节AsA再生.
过度表达FaMDHAR50增加了38%的AsA含量,提高了AsA生物合成/循环基因的调节,改变了糖和有机酸的特征,并影响了氨酸含量.

结论:

FaMDHAR50是草果中的AsA再生的关键正调节基因.
在成熟过程中,FAMDHAR50会影响草果的味道,外观和质地.
这项研究提供了关于草中AsA代谢和水果质量的遗传调节的见解.

关键词:

在FAMMDHAR50中使用.安托西亚宁的积累阿斯科布酸再生再生水果质量水果的质量.进行全基因组分析.草草是一种糖和酸代谢的代谢过程

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