炎症性介质干细胞表达丰富的膜结合和可溶性形式的C型单蛋白类CD248的CD248
Melissa Payet1, Franck Ah-Pine1,2, Xavier Guillot1,3
1Unité de Recherche en Pharmaco-Immunologie (EPI), Université et CHU de La Réunion, 97400 Saint-Denis, France.
International journal of molecular sciences
|June 10, 2023
概括
在类风湿性关节炎 (RA) 中,可溶性CD248 (sCD248) 通过矩阵金属蛋白酶 (MMP) 从炎症性介质干细胞 (MSC) 释放出来. 这种由细胞因子触发的分泌表明sCD248有助于RA的发病.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- CD248是一种在介酶干细胞 (MSC) 上发现的糖蛋白标记物.
- 它在类风湿性关节炎 (RA) 等炎症性疾病中的作用尚未完全理解.
- 了解CD248调节对于RA病变研究至关重要.
研究的目的:
- 研究介质干细胞中CD248表达和脱落的调节.
- 确定细胞因子和生长因子在CD248调节中的作用.
- 探索CD248及其可溶性形式在类风湿性关节炎中的潜在参与.
主要方法:
- 在体外分析CD248表达,使用皮肤和突介质干细胞系.
- 用各种细胞因子 (IL1-β,TNF-α,IFN-γ) 和生长因子 (VEGF,bFGF,TGF-β1,PMA) 治疗的细胞.
- 在患者样本和细胞培养超级生物中量化可溶性CD248 (sCD248) 度;评估了MMPs的mRNA表达.
主要成果:
- 在刺激后,膜结合的CD248表达没有显著变化.
- 在IL1-β和PMA治疗后检测到可溶性CD248 (sCD248).
- 矩阵金属蛋白酶 (MMPs) MMP-1和MMP-3被IL1-β和PMA上调,一个MMP抑制剂阻断了sCD248的释放.
- 在RA突发性关节组织中,在CD90+周周血管MSC上发现了CD248,与VEGF同定位.
- 在RA患者的突流体中观察到较高的sCD248水平.
结论:
- CD248通过炎症性MSCs表达,并以依赖于矩阵金属蛋白酶的方式脱落.
- 细胞因子和亲血管性因素诱导CD248分泌.
- 膜结合和溶解的CD248都可能在类风湿性关节炎的发病过程中发挥作用.
关键词:
CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD248 CD249 CD248 CD248 CD249 CD248 CD248 CD249 CD248 CD248 CD248 CD249 CD2 is what you see is what you see is what you see is what you see is what you see is这是一种炎症炎症炎症炎症.介质细胞干细胞介质细胞干细胞类风湿性关节炎 类风湿性关节炎
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