在Medicago sativa中,SPL9在酸盐充足条件下调节结节
Vida Nasrollahi1,2, Gamalat Allam1,2, Susanne E Kohalmi2
1Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V 4T3, Canada.
International journal of molecular sciences
|June 10, 2023
概括
草促进体结合蛋白类型9 (SPL9) 调节草结结的反应酸盐. 沉默SPL9会增加结节,而过度表达会影响对酸盐敏感的基因,影响这种重要的料作物的固.
科学领域:
- 植物分子生物学 植物分子生物学
- 豆类 - 树枝植物共生体
- 农业科学 农业科学
背景情况:
- 豆类中的结节,如 (Medicago sativa) 对于固定至关重要,但特征的育种是不发达的.
- 由miR156准的squamosa-Promoter Binding Protein-Like 9 (SPL9) 正在研究其在花结节中的作用.
研究的目的:
- 研究MsSPL9在调节中结节的功能,特别是在应对不同的可用性方面.
- 了解MsSPL9影响酸盐敏感基因和结节的分子机制.
主要方法:
- 生成的转基因草与沉默 (SPL9-RNAi) 和过度表达 (35S::SPL9) MsSPL9.
- 在不同的条件下,转基因植物和野生植物 (WT) 的结节表型进行比较.
- 分析了对酸盐敏感基因 (NR1,NR2,NRT2.5) 和AON基因 (SUNN) 的转录水平.
主要成果:
- 在中抑制MsSPL9显著增加了结节的形成.
- 在高酸盐条件下,MsSPL9通过调节NR1,NR2,NR2.5和SUNN基因表达来调节结节.
- MsSPL9的过度表达增加了这些基因的转录水平,而沉默导致了下调和缺乏的表型.
结论:
- MsSPL9在调节木结节的过程中起着关键作用,以应对酸盐的存在.
- 了解 MsSPL9 的功能有助于提高的使用效率.
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