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DNA-黄金纳米粒子结合方法的比较:在侧流核酸生物传感器中的应用

Qiaoling Ding^1,2,3, Wanwei Qiu^1,4, Chunxue Sun^1,2,3

¹School of Food Engineering, Anhui Science and Technology University, Fengyang 233100, China.

Molecules (Basel, Switzerland)

|June 10, 2023

概括

丁醇脱水方法为制备DNA-金纳米粒子 (DNA-AuNP) 结合物提供了一种优越的方法,显著提高了侧流核酸生物传感器 (LFNAB) 的灵敏度. 与传统技术相比,这种方法实现了100倍低的检测极限.

科学领域:

生物传感器技术的技术
纳米粒子结合的结合方法
核酸检测检测核酸检测的方法

背景情况:

侧流核酸生物传感器 (LFNAB) 对于快速,经济高效和视觉解释的诊断非常有价值.
准备DNA-金纳米粒子 (DNA-AuNP) 合物对于LFNAB敏感性至关重要.
现有的DNA-AuNP结合方法包括盐老化,微波辅助干燥加热,冷解和低pH技术.

研究的目的:

用五种不同的DNA-AuNP结合方法制备的LFNABs的分析性能进行比较分析.
确定最佳的结合方法,以实现LFNABs的高灵敏度.
为了证明优化的LFNAB在检测人类血清中的miRNA-21中的应用.

主要方法:

用五种不同的方法制备DNA-AuNP结合物:盐老化,微波辅助干燥加热,冷解,低pH和butanol脱水.
使用每个方法的DNA-AuNP结合物制造和分析测试LFNABs.
系统优化丁醇脱水方法用于DNA-AuNP结合.

主要成果:

丁醇脱水方法产生了LFNAB,在评估的五种方法中,其检测极限最低.
使用butanol脱水方法优化LFNABs实现了单链DNA的5 pM的检测极限,比盐老化方法提高100倍.
开发的LFNAB在人类血清样本中成功检测出miRNA-21.

结论:

丁醇脱水方法是一种快速有效的结合策略,用于为LFNABs制备高性能DNA-AuNP结合物.
这种方法显著提高了LFNAB的灵敏度,使得检测极限更低.
丁醇脱水方法有可能在DNA生物传感器和其他生物医学领域得到更广泛的应用.

关键词:

它们是DNA DNA DNA DNA.丁醇脱水脱水丁醇脱水黄金纳米粒子是什么意思侧向流动的侧向流动核酸生物传感器是一个核酸生物传感器.

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