使用固相微提取和气相色谱-质谱检测挥发性有机化合物的方法,用于呼吸活检
Eray Schulz1,2, Mark Woollam1,2, Paul Grocki2
1Department of Chemistry and Chemical Biology, Indiana University-Purdue University, Indianapolis, IN 46202, USA.
Molecules (Basel, Switzerland)
|June 10, 2023
概括
对挥发性有机化合物 (VOC) 的呼吸采样方法进行比较,冷转移技术为大多数VOC提供了最高的灵敏度. 然而,Tedlar-SPME在小VOC如乙方面表现出色,而直接呼吸的SPME提供了快速的结果.
科学领域:
- 分析化学 分析化学
- 生物标志物发现发现
- 呼吸系统医学 呼吸系统医学
背景情况:
- 呼气中的挥发性有机化合物 (VOC) 是代谢的副产品和潜在的疾病生物标志物.
- 气色谱-质谱 (GC-MS) 是VOC分析的黄金标准,需要有效的采样技术.
- 固态微提取 (SPME) 是一种多用途的VOC采样方法.
研究的目的:
- 开发和比较新的和现有的采样和预先缩呼气中的VOC的方法.
- 为了评估直接呼吸SPME (DB-SPME),Tedlar-袋SPME (Tedlar-SPME) 和冷转移方法的性能.
- 为不同的VOC和分析需求确定最佳的呼吸采样策略.
主要方法:
- 开发和优化内部直接呼吸SPME (DB-SPME) 方法.
- 使用GC-MS/QTOF的DB-SPME与Tedlar-SPME和冷转移方法的定量比较.
- 对呼吸样本 (每种方法n=15) 进行针对性VOC的分析,包括乙,异二烯,多二烯,二烯和二烯.
主要成果:
- 结晶转移方法对大多数VOC的总体灵敏度最高.
- 泰德拉-SPME为亚和异烯等低分子量挥发性有机物提供了更高的灵敏度.
- DB-SPME的灵敏度较低,但提供了快速分析和最小的GC-MS背景噪声.
结论:
- 这三种方法都能有效地检测出口气中的各种VOCs.
- 低温转移对于大型样本集和长期储存在-80°C是最优的.
- 泰德拉-SPME对小VOC有效,而DB-SPME适合立即分析.
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