在水浸压力下,在两种繁殖方法 (切割和移植) 内的三种木种植物中,对光合作用的生理和转录组分析
1Cash Crops Research Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
Plants (Basel, Switzerland)
|June 10, 2023
概括
浸水会对木植物产生负面影响,减少生长和光合作用. 与移植相比,切割传播显示出更好的弹性和从水应激中恢复,为改善木遗传提供了洞察力.
科学领域:
- 植物科学 植物科学
- 农业科学 农业科学
- 遗传学 是一个遗传学.
背景情况:
- 摩尔 (Morus spp.) 是一种葡萄树. 是一个经济上重要的木质植物.
- 浸水严重影响了树的生长和生产力.
- 传播方法,切割和移植,可能会影响应激反应.
研究的目的:
- 为了研究被水淹没的木品种的生理和基因表达反应.
- 为了比较水浸对通过切割与接种传播的木的影响.
- 为了确定有助于木水浸耐受性的遗传因素.
主要方法:
- 评估的生理参数包括叶绿素,蛋白质,糖,素,MDA和酶活动 (APX,POD,CAT,SOD).
- 测量的光合作用参数:净光合作用 (Pn),口腔导电率 (Gs) 和透气率 (Tr).
- 使用RNA测序分析基因表达模式,识别差异表达基因 (DEG) 并进行GO/KEGG丰富分析.
主要成果:
- 浸水减少了所有品种的关键生理和光合作用参数.
- 在水浸下切割和接种组之间没有观察到显著的生理差异.
- 基因表达分析显示了显著的变化,光合作用相关的基因下调,但在切片的第10天上调,特别是碳固定基因.
结论:
- 与接种相比,切割传播显示了与水浸压力相比的优越恢复能力.
- 基因表达模式突出显示了在传播方法之间对积水的不同反应.
- 这些发现提供了有价值的遗传信息,可以通过育种计划提高木的浸水耐受性.
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